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Rabbit Anti-SMPD2 Polyclonal Antibody#abs135820

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Description

Catalog-specification	Delivery time	USD price
abs135820-100ug	1-2 Weeks	301.0
abs135820-50ug	1-2 Weeks	201.0

Overview
catalog	abs135820
Description	Sphingomyelinases (SMases) are enzymes that catalyze the breakdown of sphingomyelin to produce ceramide and phosphocholine. Ceramide is a bioactive lipid that plays a crucial role in cell proliferation, apoptosis, and differentiation. The various types of SMases are classified based on their pH sensitivity, cation dependence, tissue distribution, and subcellular localization. These include lysosomal acid SMase, Zn++-dependent secreted acid SMase, membrane-bound Mg++-dependent neutral SMase, Mg++-independent neutral SMase, and alkaline SMase. Among them, nSMase1 (also known as SMPD2) is a Mg++-dependent neutral SMase that is widely found in cells and predominantly located in the endoplasmic reticulum. It has been discovered that nSMase1 also possesses lyso-platelet activating factor (PAF) phospholipase C activity. Another type of neutral SMase, nSMase2 (also called SMPD3), is mainly expressed in the brain. The activity of neutral SMases is influenced by oxidative stress, chemotherapeutic drugs, inflammatory cytokines, and apoptotic signals. Studies on knockout mice lacking SMPD2 and SMPD3 genes have revealed that the absence of both genes results in the complete loss of neutral SMase activity, leading to developmental abnormalities specifically associated with the loss of nSMase2.
Other names	Lyso-platelet activating factor phospholipase C, also known as Lyso-PAF-PLC or Lyso-platelet-activating factor-phospholipase C, is an enzyme involved in the metabolism of phospholipids. It is also referred to as N-SMase or Neutral sphingomyelinase. In humans, it is encoded by the NSMA_HUMAN gene and is sometimes called nSMase, NSMASE1, or Smpd2. This enzyme, Sphingomyelin phosphodiesterase 2, plays a crucial role in the breakdown of sphingomyelin, a major component of cell membranes. It catalyzes the hydrolysis of sphingomyelin into ceramide and phosphorylcholine, leading to the generation of lipid messengers with bioactive properties. Sphingomyelin phosphodiesterase 2 is primarily localized to the plasma membrane, where it cleaves sphingomyelin molecules. It is a key player in various cellular processes, including cell signaling, apoptosis, inflammation, and cell proliferation. Understanding the function and regulation of Lyso-platelet activating factor phospholipase C has important implications in different fields, such as drug discovery and the development of therapies targeting lipid metabolism. Further research into the roles and mechanisms of this enzyme will contribute to advancing our knowledge of cellular processes and the treatment of related diseases.
Source	Rabbit
Specificity	The SMPD2 Antibody is designed to detect total SMPD2 levels that occur naturally within the body. By targeting endogenous levels of SMPD2, this antibody is a valuable tool for researchers seeking to better understand the role of this protein in various physiological processes. Whether used in vitro or in vivo, the SMPD2 Antibody offers reliable and accurate detection of this important protein.
Species Reactivity	Human;Mouse;Rat
Antigen	SMPD2
Application	The dilution ranges for Western blotting (WB) are 1:500-1:2000, for immunohistochemistry (IHC) are 1:50-1:200, and for ELISA (peptide) are 1:20000-1:40000.
Immunogen	A synthesized peptide derived from human SMPD2.
MW	48kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF.
Celluar localization	Plasma Membrane;
UniPort	O60906