Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-IRAK4 Polyclonal Antibody#abs133046

Please note that the price mentioned above is only for your reference. For the actual price details, it is recommended to get in touch with our seller Vecent. Kindly arrange for a consultation with Vecent to get more information on the pricing. Western blot analysis of IRAK4 expression in A431...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs133046-50ug	1-2 Weeks	201.0
abs133046-100ug	1-2 Weeks	301.0

Overview
catalog	abs133046
Description	Efficient recruitment of IRAK1 to the IL-1 receptor complex is crucial upon IL-1 engagement in order to initiate intracellular signaling pathways. This recruitment triggers a series of events, including transcriptional up-regulation and mRNA stabilization. One of the key steps in this process is the phosphorylation of IRAK1. By phosphorylating IRAK1, the IL-1 receptor complex ensures its activation and subsequent participation in the signaling cascades. These cascades eventually lead to the desired outcomes such as gene expression changes and cellular responses.
Other names	Interleukin 1 receptor associated kinase 4 (IL-1R4), also known as IRAK-4, is a protein associated with IL-1 receptor signaling. Mutations in IRAK4 gene can lead to a mutant form of IRAK-4. Additionally, this protein is also referred to as IPD1, NY REN 64 antigen, or NY-REN-64, as it has been implicated in renal carcinoma. The gene responsible for encoding IRAK-4 is designated as LOC 51135. Overall, IRAK-4 plays a crucial role in IL-1 receptor-mediated signaling pathways and is of significant interest in various research fields.
Source	Rabbit
Specificity	The total IRAK4 can be detected by the IRAK4 antibody, which is capable of identifying the endogenous levels of this protein.
Species Reactivity	Human;Mouse;Rat
Antigen	IRAK4
Application	The dilution ratios for Western blot (WB) range from 1:500 to 1:2000, for immunohistochemistry (IHC) range from 1:50 to 1:200, and for ELISA (peptide) range from 1:20000 to 1:40000.
Immunogen	A synthesized peptide.
MW	51 kDa;
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, peptide affinity chromatography was employed to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Make sure to store at a temperature of -20 °C for a duration of one year without subjecting it to repeated freeze/thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Target
Background	Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.
Posttranslational modification	Phosphorylated.
Celluar localization	Cytosol;Endosome;Extracellular region or secreted;Nucleus;Plasma Membrane;
UniPort	Q9NWZ3