Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-SIX1 Polyclonal Antibody#abs141208

Please note that the price provided is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent. We encourage you to reach out for accurate and up-to-date pricing details. Western blot analysis of extracts from NIH/3T3 cells, using SIX1 antibody....

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs141208-100ug	1-2 Weeks	301.0
abs141208-50ug	1-2 Weeks	201.0

Overview
catalog	abs141208
Other names	SIX1 is a homeobox protein that is associated with two genetic disorders, BOS3 and DFNA23. It is also known by other names such as OTTHUMP00000179042, Sine oculis homeobox homolog 1, SIX homeobox 1, and TIP39. This protein plays an important role in embryonic development and has been found to be aberrantly expressed in certain cancers. The presence of SIX1 in BOS3 and DFNA23 suggests its involvement in the development of these disorders. Homeobox proteins are a group of transcription factors that regulate gene expression during embryonic development. SIX1, in particular, is known to be essential for the formation of several organs and structures including the eyes, ears, and kidneys. In addition to its developmental role, SIX1 has also been implicated in cancer progression and metastasis. Studies have shown that increased expression of SIX1 is associated with poor prognosis in various types of cancers, including breast, ovarian, and prostate cancer. It promotes tumor growth by enhancing cell proliferation, survival, and angiogenesis. Targeting SIX1 has emerged as a potential therapeutic strategy for cancer treatment. By inhibiting its activity or blocking its expression, it may be possible to suppress tumor growth and improve patient outcomes. However, more research is needed to better understand the molecular mechanisms underlying the role of SIX1 in cancer and to develop effective targeted therapies. In conclusion, SIX1 is a homeobox protein with important functions in embryonic development and cancer progression. Its abnormal expression is associated with genetic disorders and various types of cancers. Investigating the mechanisms of SIX1 could provide insights into both developmental biology and cancer biology, ultimately leading to the development of novel therapeutic approaches.
Source	Rabbit
Specificity	Total SIX1 levels are detected by the SIX1 Antibody, which specifically targets endogenous proteins. The antibody's ability to recognize and quantify endogenous SIX1 ensures accurate measurements of its presence.
Species Reactivity	Human;Mouse
Predictive reaction species	Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse;
Antigen	SIX1
Application	For Western blotting, the recommended dilution range is 1:500 to 1:1000. For immunofluorescence and immunocytochemistry, a dilution range of 1:100 to 1:500 is suitable. For ELISA using a peptide antigen, a dilution range of 1:20000 to 1:40000 is appropriate. Please note that these dilution ranges are based on the original text information and should be followed carefully for best results.
Immunogen	A synthesized peptide.
MW	33 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification of the antiserum was achieved through peptide affinity chromatography. Let me rephrase the statement in a different manner while retaining the essential information: The SulfoLink™ Coupling Resin was employed for peptide affinity chromatography, leading to the purification of the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain its quality, store at a temperature of -20 °C without subjecting it to freeze/thaw cycles for a period of one year. It is crucial to avoid repeated freeze/thaw cycles to ensure its longevity.
Storage buffer	This product is Rabbit IgG that is supplied in a pH 7.4 phosphate buffered saline solution containing 150mM NaCl, 0.02% sodium azide and 50% glycerol. It is recommended that this product be stored at a temperature of -20°C for optimal preservation and functionality. The product is stable for a duration of 12 months from the date of receipt.
Target
Background	Transcription factor that is involved in the regulation of cell proliferation, apoptosis and embryonic development. Plays an important role in the development of several organs, including kidney, muscle and inner ear. Depending on context, functions as transcriptional repressor or activator. Lacks an activation domain, and requires interaction with EYA family members for transcription activation. Mediates nuclear translocation of EYA1 and EYA2. Binds the 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the MYOG promoter. Regulates the expression of numerous genes, including MYC, CCND1 and EZR. Acts as activator of the IGFBP5 promoter, probably coactivated by EYA2. Repression of precursor cell proliferation in myoblasts is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex. During myogenesis, seems to act together with EYA2 and DACH2 (By similarity). Regulates the expression of CCNA1.
Tissue specificity	Specifically expressed in skeletal muscle.
Posttranslational modification	Phosphorylated during interphase; becomes hyperphosphorylated during mitosis. Hyperphosphorylation impairs binding to promoter elements.Ubiquitinated by the anaphase promoting complex (APC), leading to its proteasomal degradation.
Celluar localization	Nucleus;
UniPort	Q15475