Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-SIRT4 Polyclonal Antibody#abs137953

Please note that the mentioned price is only for your information. For detailed pricing, kindly get in touch with our seller, Vecent. We encourage you to contact Vecent for accurate and up-to-date pricing information. Western blot analysis SIRT4 using Jurkat whole cell lysates This product is...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs137953-100ug	1-2 Weeks	301.0
abs137953-50ug	1-2 Weeks	201.0

Overview
catalog	abs137953
Other names	Sirtuin 4, also known as SIRT4, is a NAD-dependent protein deacetylase that belongs to the sirtuin family. It is encoded by several genes, including MGC130046, MGC130047, MGC57437, and SIR2L4. Sirtuin 4 is a mitochondrial protein involved in various cellular processes such as metabolism, insulin secretion, and stress response. The protein has been shown to act as a regulator of energy metabolism, particularly in glucose and fatty acid metabolism. Additionally, it has been implicated in the maintenance of mitochondrial integrity and the protection against oxidative stress. Sirtuin 4 has also been associated with the regulation of lifespan and age-related diseases, like cancer and neurodegeneration. Overall, Sirtuin 4 has emerged as a promising target for therapeutic interventions aimed at improving metabolic health and reducing the risk of age-related diseases. Research on the role and regulation of Sirtuin 4 is ongoing, and advances in this field have the potential to advance our understanding of fundamental biological processes and their relevance to human health.
Source	Rabbit
Specificity	The SIRT4 Antibody is designed to recognize total SIRT4 levels in endogenous samples. The antibody can accurately detect the expression of the target protein.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Pig;Dog;Horse;Bovine
Antigen	SIRT4
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human SIRT4.
MW	35 kDa
Properties
Concentration	1mg/ml
purification	Utilizing SulfoLink™ Coupling Resin, the antiserum underwent purification via peptide affinity chromatography. To ensure high quality, this process was carefully executed.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner (PubMed:25525879). Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest (PubMed:16959573, PubMed:17715127). In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor (PubMed:23562301, PubMed:23663782). Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis (By similarity). Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1:PPARA interaction probably through the regulation of NAD+ levels (PubMed:24043310). Down-regulates insulin secretion.
Tissue specificity	Detected in vascular smooth muscle and striated muscle. Detected in insulin-producing beta-cells in pancreas islets of Langerhans (at protein level). Widely expressed. Weakly expressed in leukocytes and fetal thymus.
Celluar localization	Mitochondrion;
UniPort	Q9Y6E7