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Rabbit Anti-RNF5 Polyclonal Antibody#abs139010

Please note that the price provided is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent. Western blot analysis RNF5 using Jurkat whole cell lysates This product is for research use only, not for use in diagnostic prodecures or in human.

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Description

Catalog-specification	Delivery time	USD price
abs139010-100ug	1-2 Weeks	301.0
abs139010-50ug	1-2 Weeks	201.0

Please note that the price provided is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent.

Overview
catalog	abs139010
Other names	The RNF5 protein, also known as RING finger protein 5, G16, NG2, or HsRma1, is an E3 ubiquitin-protein ligase that plays a critical role in ubiquitination and subsequent degradation of target proteins. This protein is encoded by the RNF5 gene and is highly conserved across different species. RNF5 contains a RING finger domain that binds with specific E2 ubiquitin-conjugating enzymes, facilitating the transfer of ubiquitin to the target protein. RNF5 has been implicated in multiple cellular processes including endocytosis, protein trafficking, and immune response. Additionally, RNF5 is involved in the regulation of protein turnover, enhancing the efficiency of the ubiquitin-proteasome system. Overall, the importance of RNF5 in regulating cellular functions underscores its potential as a therapeutic target.
Source	Rabbit
Specificity	The RNF5 Antibody is capable of detecting the total endogenous levels of RNF5. To create similar content in a different format, the information can be stated as follows: The RNF5 Antibody can identify the complete amount of RNF5 present naturally within a cell.
Species Reactivity	Human;Mouse;Rat
Antigen	RNF5
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human RNF5.
MW	20 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized in the process of peptide affinity chromatography to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage conditions, it is recommended to store at a temperature of -20 °C for a period of one year. Repeated freeze/thaw cycles should be avoided to maintain the integrity of the product.
Storage buffer	This product is rabbit IgG dissolved in phosphate-buffered saline (PBS) with a pH of 7.4. It also contains 150mM of NaCl, 0.02% sodium azide, and 50% glycerol. For storage, it is recommended to keep it at -20°C. The product remains stable for a period of 12 months from the date of receipt.
Target
Background	Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurs in mitochondria after viral transfection and regulates antiviral responses.
Tissue specificity	Widely expressed.
Celluar localization	Endoplasmic reticulum;Mitochondrion;
UniPort	Q99942