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Rabbit Anti-AGTR1 Polyclonal Antibody#abs135212

Please note that the price mentioned is for reference purposes only. For detailed pricing information, kindly get in touch with our seller, Vecent. We recommend reaching out to Vecent for accurate pricing details. Western blot analysis of extracts from K562 cells, using AGTR1 antibody. This...

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Description

Catalog-specification	Delivery time	USD price
abs135212-100ug	1-2 Weeks	301.0
abs135212-50ug	1-2 Weeks	201.0

Overview
catalog	abs135212
Other names	The human angiotensin II receptor type 1, also known as AGTR1, AGTR1_HUMAN, or Agtr1a, is an important protein involved in the renin-angiotensin-aldosterone system. It plays a crucial role in regulating blood pressure and fluid balance in the body. The receptor is stimulated by angiotensin II, a hormone that constricts blood vessels and releases aldosterone, leading to increased sodium reabsorption and water retention. The angiotensin II receptor type 1 is encoded by the AGTR1 gene and can exist in two isoforms, named Agtr1a and Agtr1b. These isoforms have distinct signaling properties and tissue distribution. Agtr1a is mainly found in the heart, blood vessels, and kidneys, while Agtr1b is more widely expressed in various tissues. The receptor is also known as AT1 as it belongs to the AT1 receptor family. Other aliases for the angiotensin II receptor type 1 include AT1AR, AT1B, AT1BR, AT1R, AT2R1, AT2R1A, AT2R1B, HAT1R, and Type-1 angiotensin II receptor. The AT1 receptor is responsible for mediating the vasoconstrictive effects of angiotensin II, leading to increased blood pressure. Understanding the structure and function of the angiotensin II receptor type 1 is crucial for developing targeted therapies for cardiovascular diseases such as hypertension and heart failure. Researchers are continually investigating the signaling mechanisms and interactions of this receptor to explore new treatment options and improve patient outcomes.
Source	Rabbit
Specificity	The AGTR1 Antibody recognizes total AGTR1 at endogenous levels. This means that it can detect the presence of AGTR1 in natural levels within a sample.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Sheep;Dog;Bovine;
Antigen	AGTR1
Application	For WB (western blotting), the recommended dilution of the primary antibody is 1:500-1:1000. For IF/ICC (immunofluorescence/immunocytochemistry) experiments, a dilution of 1:100-1:500 is suggested. When performing IHC (immunohistochemistry) staining, a recommended dilution of 1:100-200 should be used. Finally, for ELISA (enzyme-linked immunosorbent assay) using a peptide, a recommended dilution range of 1:20000-1:40000 is suggested for optimal results. It's important to adhere to the recommended dilutions to ensure proper detection and to minimize background noise.
Immunogen	A synthesized peptide.
MW	41 KD
Properties
Concentration	1mg/ml
purification	Utilizing SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. To produce highly similar content, this information can be rearranged as follows: The purification process of the antiserum involved utilizing SulfoLink™ Coupling Resin through peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure proper storage, it is recommended to keep the product at a temperature of -20 °C for up to one year. It is important to avoid exposing the product to repeated freeze/thaw cycles, as this may compromise its quality. Proper storage is essential to maintain the integrity of the product and to ensure that it remains effective for its intended purpose. Take care to follow these guidelines to ensure that your product remains in optimal condition.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Tissue specificity	Liver, lung, adrenal and adrenocortical adenomas.
Posttranslational modification	C-terminal Ser or Thr residues may be phosphorylated.
Celluar localization	Plasma Membrane;
UniPort	P30556