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Rabbit Anti-RNF123 Polyclonal Antibody#abs139008

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Description

Catalog-specification	Delivery time	USD price
abs139008-100ug	1-2 Weeks	301.0
abs139008-50ug	1-2 Weeks	201.0

Overview
catalog	abs139008
Other names	RNF123, also known as KPC1 or Kip1 ubiquitination-promoting complex protein 1, is an E3 ubiquitin protein ligase that plays an important role in the ubiquitination process. It belongs to the RING finger protein family and is involved in the regulation of protein degradation. FLJ12565 and MGC163504 are also aliases of this protein. The function of RNF123 is to tag proteins with ubiquitin molecules, which leads to their degradation by the proteasome. This protein is involved in the ubiquitin-mediated proteolysis of p27 Kip1, a cyclin-dependent kinase inhibitor that regulates the cell cycle. RNF123 contains a RING finger domain, which is essential for its E3 ligase activity. It has been reported that RNF123 is involved in the development of breast cancer and glioma, indicating its potential as a therapeutic target for cancer treatment. In summary, RNF123 is a crucial ubiquitin ligase that plays an important role in the regulation of protein degradation. Its functions are related to the processes of the cell cycle and cancer development, making it an important target for future research.
Source	Rabbit
Specificity	Total RNF123 can be detected by the RNF123 Antibody, which is capable of detecting the endogenous levels. The content, although highly similar, is rearranged to convey the same information regarding the detection of total RNF123 using the RNF123 Antibody.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Zebrafish;Chicken;Rabbit;Xenopus;Dog;Horse;Bovine;Sheep;Pig
Antigen	RNF123
Application	The recommended dilutions for WB are 1:1000-3000, for IHC are 1:200, and for ELISA using a peptide are 1:20000-1:40000. Please note that the dilutions may vary depending on the specific experiment and should be optimized accordingly.
Immunogen	A synthesized peptide derived from human RNF123.
MW	149 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed to achieve purification of the antiserum through peptide affinity chromatography. The process involved the rearrangement of the original content for optimal results.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality, store at a temperature of -20 °C for a duration of one year. It is crucial to prevent repeated freeze/thaw cycles. It is advised to carefully follow this storage guideline to ensure optimal preservation.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.
Posttranslational modification	Ubiquitinated, leading to its degradation. Deubiquitinated by USP19, thereby stimulating CDKN1B ubiquitin-dependent degradation.
Celluar localization	Cytosol;
UniPort	Q5XPI4