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Rabbit Anti-RHOC Polyclonal Antibody#abs135727

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Description

Catalog-specification	Delivery time	USD price
abs135727-100ug	1-2 Weeks	301.0
abs135727-50ug	1-2 Weeks	201.0

Overview
catalog	abs135727
Description	The Rho family of small GTPases, which includes Rho, Rac, and cdc42, plays a vital role in regulating critical cellular processes such as cell migration, adhesion, proliferation, and differentiation. These proteins act as molecular switches, and their activity is controlled by guanine nucleotide exchange factors (GEFs), which catalyze the conversion of bound GDP to GTP. Conversely, GTPase activating proteins (GAPs) facilitate the hydrolysis of GTP to GDP, thereby inhibiting the activity of these proteins. The function of Rho family small GTPases is further regulated by Rho GDP dissociation inhibitor (RhoGDI), which acts by stoichiometrically binding to these proteins. Of the three highly homologous Rho proteins, RhoA, RhoB, and RhoC, distinct differences in their C-terminal modifications and subcellular localization endow them with divergent biological functions. These differences enable them to respond to and act on different signaling molecules, which are crucial for the regulation of cellular processes. Overall, the Rho family of small GTPases plays an essential role in regulating cellular processes, and their activity is tightly controlled at multiple levels.
Other names	Genes, such as ARH12, ARHA, and H12, belong to the ras homolog gene family. Other members of this family include Rho cDNA clone 12, RHO12, RHOA, RHOA_HUMAN, rhob, rhoc, and RHOH12. They are all small GTP binding proteins known as Ras homologs. One prominent member of this family is the protein RhoA, also referred to as Transforming protein RhoA. It plays a crucial role in various cellular processes by regulating cytoskeletal reorganization and cell shape. RhoA is known for its involvement in cell migration, adhesion, and cytokinesis. The presence of RhoA indicates the essential role it plays in cell physiology and its indispensability in maintaining cellular homeostasis. The intricate interplay of RhoA and other members of the Ras homolog gene family highlights their significance in various biological processes. By rearranging the provided content, we gain a better understanding of the diverse roles played by these genes and their implications in cellular function and regulation.
Source	Rabbit
Specificity	The RHOC Antibody is designed to detect the presence of total RHOC at its natural levels. The antibody can bind to endogenous RHOC, allowing researchers to accurately measure the amount of RHOC present in samples. This capability enables researchers to understand the biological function and role of RHOC in various cellular processes.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Bovine;Dog;Horse;
Antigen	RHOC
Application	The dilutions for Western Blot are 1:500-1:2000, for Immunohistochemistry are 1:50-1:200, and for ELISA using peptide are 1:20000-1:40000. Please rearrange the information above to create similar content based on the original text.
Immunogen	A synthesized peptide derived from human RHOC.
MW	22kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Regulates apical junction formation in bronchial epithelial cells.
Posttranslational modification	(Microbial infection) Glycosylated at Tyr-34 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rho and leads to actin disassembly.
Celluar localization	Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;
UniPort	P08134