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Rabbit Anti-PSMD4 Polyclonal Antibody#abs135726

Please note that the price provided above is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent. Western blot analysis of K562 cell and jurkat cell lysate using PSMD4 antibody. This product is for research use only, not for...

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Description

Catalog-specification	Delivery time	USD price
abs135726-100ug	1-2 Weeks	301.0
abs135726-50ug	1-2 Weeks	201.0

Please note that the price provided above is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent.

Overview
catalog	abs135726
Description	S5a (PSMD4) is a pivotal component of the 19S regulatory proteasome complex, which plays a crucial role in the recognition and degradation of ubiquitinated proteins (1). With its two polyubiquitin binding motifs (UIM), S5a can selectively bind to multiubiquitin chains through hydrophobic interaction (2,3). Interestingly, the UIM of S5a exhibits a remarkable affinity not only for ubiquitin but also for a ubiquitin-like domain found in various proteins. By directly interacting with these proteins, S5a serves as a crucial mediator in directing them towards the proteasome for subsequent degradation (4,5).
Other names	Proteasome subunit S5a, also known as 26S proteasome regulatory subunit rpn10 or multisubstrate protein ASF, is a non-ATPase regulatory subunit of the 26S proteasome. It has been implicated in several cellular processes, including protein degradation, cell division, and transcriptional regulation. S5a is a multiubiquitin chain-binding protein that recognizes and binds to ubiquitinated proteins, targeting them for proteasomal degradation. This process plays a critical role in maintaining cellular homeostasis by regulating the turnover of damaged or misfolded proteins. In addition to its role in protein degradation, S5a has also been shown to interact with and regulate the activity of several transcription factors, including P53 and NF-κB. These interactions suggest that S5a may play a broader role in the regulation of gene expression. Overall, S5a is a versatile protein that plays a critical role in maintaining cellular homeostasis and regulating key cellular processes. Further research on the functions of this protein will undoubtedly reveal even more insights into its importance in cellular biology.
Source	Rabbit
Specificity	Total PSMD4 can be detected by the PSMD4 Antibody at endogenous levels. Let me create a similar content by rearranging the information in a different manner: The PSMD4 Antibody is capable of detecting the overall expression levels of endogenous PSMD4.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep
Antigen	PSMD4
Application	The recommended dilutions for using the primary antibody in various assays are as follows: for Western blotting, use a dilution range of 1:500 to 1:2000; for immunohistochemistry, use a dilution range of 1:50 to 1:200; for immunofluorescence and immunocytochemistry, use a dilution range of 1:100 to 1:500; and for ELISA using a peptide, use a dilution range of 1:20000 to 1:40000. It is important to ensure proper dilution for each application in order to achieve optimal results.
Immunogen	A synthesized peptide derived from human PSMD4.
MW	41kDa, 52kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.
Celluar localization	Cytosol;Nucleus;
UniPort	P55036