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Rabbit Anti-REC8 Polyclonal Antibody#abs141234

Please note that the price provided is for reference only. To obtain the detailed pricing information, we recommend that you get in touch with our seller, Vecent. Western blot analysis of extracts from K562 cells, using REC8 antibody. This product is for research use only, not for use in...

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Description

Catalog-specification	Delivery time	USD price
abs141234-100ug	1-2 Weeks	301.0
abs141234-50ug	1-2 Weeks	201.0

Please note that the price provided is for reference only. To obtain the detailed pricing information, we recommend that you get in touch with our seller, Vecent.

Overview
catalog	abs141234
Other names	REC8, also known as Cohesin Rec8p, is a protein that plays a crucial role in meiotic recombination and sister chromatid cohesion. It is considered the human homolog of rad21 in S. pombe. REC8 belongs to the rad21p family of phosphoproteins that are involved in maintaining the integrity of chromosomes during meiosis. The REC8 protein is similar to the yeast REC8 homolog and REC8-like 1 protein. It is responsible for facilitating meiotic recombination and ensuring proper alignment and segregation of chromosomes. This vital function is imperative for the accurate distribution of genetic material during cell division and the production of haploid cells in sexual reproduction. The REC8 protein is phosphorylated, which is essential for its activity in meiotic recombination and sister chromatid cohesion. Its homology to rad21 in S. pombe underscores its significance in maintaining chromosome stability and ensuring the fidelity of DNA replication during meiosis. In conclusion, REC8, also known as Rec8p, is a vital protein involved in meiotic recombination and sister chromatid cohesion. It shares similarities with yeast REC8 homolog and REC8-like 1, and its phosphorylation status is crucial for its function. Understanding the role of REC8 in meiosis is fundamental to comprehending the complex processes that enable the successful transmission of genetic information.
Source	Rabbit
Specificity	The REC8 Antibody is capable of recognizing the natural levels of total REC8 present within an organism. By rearranging the provided content, we can create a similar statement based on the original text.
Species Reactivity	Human
Antigen	REC8
Application	The dilution ratio for Western blot (WB) can vary from 1:500 to 1:1000, while for immunofluorescence (IF) and immunocytochemistry (ICC), it is recommended to dilute the primary antibody at a ratio of 1:100 to 1:500. On the other hand, when performing ELISA using a peptide as the antigen, the suggested dilution range for the antibody is 1:20000 to 1:40000. It is important to adhere to these dilution guidelines to achieve accurate and reliable results in these experimental techniques.
Immunogen	A synthesized peptide.
MW	62 KD
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography during the purification of the antiserum. Let me create a similarly-phrased content based on the original text information.
Clonality	Polyclonal Antibody
Stability & Storage	-20°C，/。
Storage buffer	This Rabbit IgG solution has been prepared in phosphate buffered saline with a pH of 7.4, 150mM of sodium chloride, and sodium azide at a concentration of 0.02%. Additionally, 50% glycerol has been added to the solution to enhance its stability during storage. To maintain the integrity of the product, it is recommended that it be stored at a temperature of -20°C. The solution is expected to remain viable for up to 12 months from the date of purchase.
Target
Background	Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II (By similarity).
Tissue specificity	Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa (at protein level).
Posttranslational modification	Phosphorylated.
Celluar localization	Nucleus;
UniPort	O95072