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Rabbit Anti-PVRL3 Polyclonal Antibody#abs134603

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Description

Catalog-specification	Delivery time	USD price
abs134603-100ug	1-2 Weeks	301.0
abs134603-50ug	1-2 Weeks	201.0

Overview
catalog	abs134603
Other names	Nectin-3, also known as Poliovirus receptor-related protein 3 (PRR3), PVRL3_HUMAN or CDw113, is a protein encoded by the PVRL3 gene. This protein is a member of the Nectin family and is involved in the formation of trans-endothelial cell tight junctions. It is also a receptor for poliovirus, which is why it is sometimes referred to as Poliovirus receptor-related 3 or Poliovirus receptor-related protein 3. Other names for Nectin-3 include DKFZP566B0846, FLJ90624, Poliovirus receptor-like 3, PPR3, and Pvrl3. With its pivotal role in the cellular tight junction formation, Nectin-3 is a crucial protein required for the proper functioning of the human body.
Source	Rabbit
Specificity	The PVRL3 Antibody is capable of detecting total PVRL3 levels present within the organism's system. This antibody is designed to determine the endogenous levels of PVRL3, providing accurate insights into the biological processes within the system. The generated content should maintain high similarity with the original text, while clarifying the key aspects discussed and offering new perspectives.
Species Reactivity	Human;Mouse
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Bovine;Horse;
Antigen	PVRL3
Application	To ensure accurate results in Western Blotting, a dilution of 1:500 to 1:1000 is recommended. When performing Immunofluorescence or Immunocytochemistry, a dilution of 1:100 to 1:500 should be used for optimal outcomes. In ELISA assays that involve the use of peptides, dilutions of 1:20000 to 1:40000 are recommended. These dilution ranges are critical for obtaining consistent and reliable results in these analytical techniques.
Immunogen	A synthesized peptide.
MW	61 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. To achieve this, the resin was linked with the appropriate peptide to target and isolate the desired substance. Subsequently, the purification process provided a highly refined form of antiserum that can be used in various experimental and diagnostic applications.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the integrity of the product, it is recommended that it be stored at a temperature of -20 °C for a period of one year. It is important to avoid exposing the product to repeated freeze/thaw cycles as this can cause damage to its properties. Proper storage conditions should be maintained to ensure the product remains effective and safe for use. Please take care to follow these guidelines closely to ensure maximum efficacy of the product.
Storage buffer	The storage conditions for Rabbit IgG include keeping it in phosphate buffered saline at a pH of 7.4. It should also be maintained in a solution containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is crucial to store it at a temperature of -20 °C to ensure its stability. This Rabbit IgG is expected to remain usable for a period of 12 months starting from the date of receipt.
Target
Background	Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.
Tissue specificity	Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.
Celluar localization	Plasma Membrane;
UniPort	Q9NQS3