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Rabbit Anti-PSME2 Polyclonal Antibody#abs136914

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Description

Catalog-specification	Delivery time	USD price
abs136914-100ug	1-2 Weeks	301.0
abs136914-50ug	1-2 Weeks	201.0

Overview
catalog	abs136914
Description	The 26S proteasome, a well-organized multicatalytic proteinase complex, consists of two main components: a 20S core and a 19S regulator. The 20S core comprises four rings, each consisting of seven non-identical subunits. Specifically, two rings consist of alpha subunits, while the other two rings consist of beta subunits. On the other hand, the 19S regulator comprises a base and a lid. The base contains six ATPase subunits and two non-ATPase subunits, while the lid consists of up to ten non-ATPase subunits. These proteasomes are found in abundance throughout eukaryotic cells and are involved in peptide cleavage via an ATP/ubiquitin-dependent mechanism, operating outside the lysosomal pathway. One important variant of the proteasome, known as the immunoproteasome, plays a critical role in processing class I MHC peptides. In contrast to the standard proteasome, the immunoproteasome replaces the 19S regulator with an alternative regulator called the 11S regulator or PA28. The 11S regulator consists of three subunits: alpha, beta, and gamma. Among these, the beta subunit encoded by this gene is induced by gamma-interferon. When combined with three alpha subunits, the beta subunit forms a heterohexameric ring, contributing to the function of the immunoproteasome. It is worth noting that several pseudogenes resembling this gene have been identified on chromosomes 4, 5, 8, 10, and 13. These non-functional gene copies do not produce proteins but can still provide insight into the evolutionary history of the genome.
Other names	The beta subunit of the 11S regulator complex, also known as PA28beta or REG-beta, is an important component of the proteasome activator complex. It is encoded by the PSME2 gene and is involved in various cellular processes. This protein has been found to play a role in cell migration and is also known as cell migration-inducing protein 22 or MCP activator 31 kD subunit. The 11S regulator complex beta subunit, or PA28beta, acts as an activator of the multicatalytic protease. It forms a complex with other subunits to enhance the proteasome activity and promote the degradation of proteins. The PA28beta subunit is part of the larger proteasome activator complex and is essential for its proper function. In summary, the 11S regulator complex beta subunit, also known as PA28beta or REG-beta, is a crucial component of the proteasome activator complex. It plays a role in cell migration and is involved in protein degradation processes. Its proper function is essential for maintaining cellular homeostasis.
Source	Rabbit
Specificity	The total PSME2 levels in the body can be effectively detected using the PSME2 Antibody, which specifically targets the endogenous PSME2 protein. To ensure accurate analysis, this antibody is designed to recognize and bind to all forms of PSME2 present in the sample. By detecting the endogenous levels of PSME2, researchers can gain valuable insights into its role and function in various biological processes. Additionally, this antibody allows for the quantification and comparison of PSME2 levels under different experimental conditions.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Zebrafish;Rabbit;Pig;Dog;Horse;Sheep;Bovine
Antigen	PSME2
Application	WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human PSME2.
MW	27kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.
Tissue specificity	By IFNG/IFN-gamma.
Celluar localization	Cytosol;Extracellular region or secreted;Nucleus;
UniPort	Q9UL46