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DRP1 Antibody#abs136557

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Description

Catalog-specification	Delivery time	USD price
abs136557-100ug	1-2 Weeks	301.0
abs136557-50ug	1-2 Weeks	201.0

Overview
catalog	abs136557
Description	DRP1 belongs to the dynamin superfamily of GTPases, which performs many cellular tasks such as organization of vesicles, splitting of organelles, managing intracellular transport, and viral resistance (1). DRP1 plays a significant role in mitochondrial and peroxisomal fission and affects mitochondrial morphology in mammalian cells (2-5). DRP1 creates a spiral-shaped structure on the mitochondrial membrane, which supports fission, similar to its yeast ortholog (6). DRP1 is phosphorylated at Ser616 by Cdk1/cyclin B and at Ser637 by PKA. Mitochondrial division, necessary for apoptosis and cell growth, relies on DRP1 phosphorylation. In mitosis, when DRP1 is phosphorylated at Ser616, it facilitates mitochondrial fission, while it is inhibited at Ser637 (6). Calcineurin reverses inhibition by dephosphorylating DRP1 at Ser637. Sumoylation of DRP1 also enhances mitochondrial fission (8). Maintaining the balance between fusion and fission is vital for proper mitochondrial function. Research indicates that neurodegenerative diseases like Alzheimer's, Parkinson's, and Huntington's affect mitochondrial function (6).
Other names	DNM1L, also known as dynamin-related protein 1, Dynamin-1-like protein, or Vps1p-like protein, is a member of the dynamin protein family. It plays a crucial role in mitochondrial fission and participates in the regulation of cellular processes such as apoptosis and autophagy. Dnm1L possesses a dynamin-like protein domain and lacks the proline-rich C-terminal domain found in other dynamin family members. It is encoded by the DNM1L gene and is also known as DVLP, DVLP11, EMPF, EMPF1, FLJ41912, and HdynIV. Dnm1L is highly conserved across species from yeast to humans and has been extensively studied in various physiological and pathological contexts.
Source	Rabbit
Specificity	The endogenous levels of total DRP1 can be detected by the DRP1 Antibody. This antibody is specifically designed to target and bind to DRP1, allowing researchers to quantify the amount of DRP1 present in a sample. By utilizing this antibody, scientists are able to study the role and regulation of DRP1 in various cellular processes.
Species Reactivity	Human;Mouse;Rat
Antigen	DRP-1
Application	WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human DRP1.
MW	79kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage. Required for normal brain development, including that of cerebellum. Facilitates developmentally regulated apoptosis during neural tube formation. Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues. Plays an important role in mitochondrial fission during mitosis (PubMed:26992161). Required for formation of endocytic vesicles. Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X(L) which stimulates its GTPase activity in synaptic vesicles; the function may require its recruitment by MFF to clathrin-containing vesicles. Required for programmed necrosis execution.
Tissue specificity	Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons.
Posttranslational modification	Phosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-637 inhibits the GTPase activity, leading to a defect in mitochondrial fission promoting mitochondrial elongation. Dephosphorylated on this site by PPP3CA which promotes mitochondrial fission. Phosphorylation on Ser-616 activates the GTPase activity and promotes mitochondrial fission.Sumoylated on various lysine residues within the B domain, probably by MUL1. Sumoylation positively regulates mitochondrial fission. Desumoylated by SENP5 during G2/M transition of mitosis. Appears to be linked to its catalytic activity.S-nitrosylation increases DNM1L dimerization, mitochondrial fission and causes neuronal damage.Ubiquitination by MARCH5 affects mitochondrial morphology.O-GlcNAcylation augments the level of the GTP-bound active form of DRP1 and induces translocation from the cytoplasm to mitochondria in cardiomyocytes. It also decreases phosphorylation at Ser-637 (By similarity).
Celluar localization	Cytoskeleton;Cytosol;Endoplasmic reticulum;Golgi apparatus;Mitochondrion;Peroxisome;
UniPort	O00429