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Rabbit Anti-PRPF3 Polyclonal Antibody#abs136876

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Description

Catalog-specification	Delivery time	USD price
abs136876-100ug	1-2 Weeks	301.0
abs136876-50ug	1-2 Weeks	201.0

Overview
catalog	abs136876
Description	Spliceosomes, complex assemblies comprising four small nuclear ribonucleoprotein particles (snRNPs) and various splicing factors, play a crucial role in the removal of introns from nuclear pre-mRNAs. Among the proteins that interact with U4 and U6 snRNPs, there exists a gene product associated with retinitis pigmentosa-18 when mutated. These mutations highlight the significance of this particular protein in the pathogenesis of retinitis pigmentosa-18.
Other names	Prp3p, also known as HPRP3P or Pre-mRNA-splicing factor 3, is a crucial component involved in the processing of precursor mRNA in organisms like S. cerevisiae. It is homologous to the Precursor mRNA-processing factor 3 and plays a vital role in splicing events. Additionally, Prp3p is recognized as U4/U6 snRNP 90 kDa protein or SNRNP90. Its significance in the pre-mRNA splicing pathway makes it an important factor in gene expression regulation. Consequently, understanding the functions and mechanisms of PRP3 and its human homolog PRPF3 (also referred to as PRPF3_HUMAN) contributes to our knowledge in identifying potential therapeutic targets for certain diseases. The PRP3 protein, encoded by the RP18 gene, exhibits high similarity to the U4/U6 small nuclear ribonucleoprotein PRP3, emphasizing its conserved role across species.
Source	Rabbit
Specificity	The PRPF3 Antibody is capable of detecting the complete levels of endogenous PRPF3. To create new content based on this information, one might phrase it as follows: The total amount of PRPF3 present in the system can be identified using the PRPF3 Antibody, which is designed to recognize endogenous levels.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Bovine;Horse;
Antigen	PRPF3
Application	The dilution ranges for the Western blot (WB) assay are 1:500 to 1:2000. For the immunohistochemistry (IHC) assay, the dilution ranges from 1:50 to 1:200. Lastly, for ELISA assays using peptides, the dilution ranges from 1:20000 to 1:40000. It is important to maintain these dilution ranges in order to obtain accurate and reliable results for each assay.
Immunogen	A synthesized peptide derived from human PRPF3.
MW	78kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized in the process of peptide affinity chromatography to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.
Tissue specificity	Highly expressed in retina, liver, kidney and blood. Detected at lower levels in heart and brain.
Posttranslational modification	Ubiquitinated. Undergoes 'Lys-63'-linked polyubiquitination by PRPF19 and deubiquitination by USP4. 'Lys-63'-linked ubiquitination increases the affinity for PRPF8 and may regulate the assembly of the U4/U6-U5 tri-snRNP complex.
Celluar localization	Cytosol;Nucleus;
UniPort	O43395