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Rabbit Anti-PP2A-C Alpha/beta Polyclonal Antibody#abs138114

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Description

Catalog-specification	Delivery time	USD price
abs138114-100ug	1-2 Weeks	301.0
abs138114-50ug	1-2 Weeks	201.0

Overview
catalog	abs138114
Other names	The protein phosphatase 2A catalytic subunit alpha isoform, known as PP2A alpha or PP2AA_HUMAN, is a crucial enzyme that plays a vital role in dephosphorylating proteins. PP2A alpha, together with its beta isoform (PP2A beta), forms the dimeric core of protein phosphatase 2A (PP2A), which is responsible for regulating many cellular processes, including cell growth, division, and signaling. PP2A alpha is encoded by the PPP2CA gene and is expressed in many tissues and cell types. It has a broad substrate specificity and can dephosphorylate various types of phosphoproteins, including transcription factors, kinases, and cytoskeletal proteins. Moreover, PP2A alpha is involved in the regulation of different pathways, such as the Wnt/beta-catenin pathway, the PI3K/Akt pathway, and the mTOR signaling pathway. The PP2A alpha isoform has also been implicated in several diseases, including cancer, Alzheimer's disease, and cardiovascular disorders. In cancer, PP2A alpha is often found to be downregulated, leading to the dysregulation of critical signaling pathways and the promotion of tumorigenesis. In Alzheimer's disease, PP2A alpha is thought to play a role in the clearance of tau protein, which forms neurofibrillary tangles in the brain. In summary, PP2A alpha is a critical enzyme involved in regulating various cellular processes, including protein dephosphorylation and signaling pathways. Its dysregulation has been associated with several diseases, highlighting the importance of understanding its function and regulation.
Source	Rabbit
Specificity	The PP2A-C alpha/β Antibody is capable of detecting overall levels of PP2A-C alpha/β present within the body. This means that the antibody is able to identify and measure the total amount of naturally occurring PP2A-C alpha/β present within a given system.
Species Reactivity	Human;Mouse;Rat
Antigen	PP2A-C alpha/β
Application	The recommended dilutions for Western blotting are between 1:1000 and 1:3000, while for immunofluorescence/immunocytochemistry, the recommended dilution range is 1:100 to 1:500. For ELISA using peptide substrates, a dilution range of 1:20000 to 1:40000 is recommended. It is important to follow these recommended dilutions to ensure reliable and accurate results in your experiments.
Immunogen	A newly synthesized peptide with a composition derived from the human PP2A-C alpha/beta protein has been developed. This peptide is similar to the original in terms of amino acid makeup and may have potential therapeutic applications in various diseases. The research team carefully monitored and optimized the production of this peptide to ensure high purity and quality. Preliminary studies suggest that this new synthesized peptide could play a crucial role in regulating cell signaling pathways and may hold promise as a future treatment option for certain types of cancer.
MW	35 kDa
Properties
Concentration	1mg/ml
purification	By employing SulfoLink™ Coupling Resin, the purification of the antiserum was achieved through peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.
Posttranslational modification	Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.May be monoubiquitinated by NOSIP.
Celluar localization	Cytoskeleton;Cytosol;Extracellular region or secreted;Mitochondrion;Nucleus;Plasma Membrane;
UniPort	P67775/P62714

This product is for research use only, not for use in diagnostic prodecures or in human.

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