Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-POLR2J Polyclonal Antibody#abs136173

Please note that the price mentioned is for reference purposes only. For detailed pricing information, please get in touch with our seller Vecent. It's important to point out that the content generated should be based on the original text, but presented in a highly similar manner with some...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs136173-100ug	1-2 Weeks	301.0
abs136173-50ug	1-2 Weeks	201.0

Overview
catalog	abs136173
Description	The RNA polymerase II subunit encoded by this gene is crucial in the process of messenger RNA synthesis in eukaryotes. It forms a core subassembly unit of the polymerase as a heterodimer along with another subunit. This gene is located on chromosome 7q22.1, and there are two similar genes in its vicinity. A pseudogene is also present on chromosome 7p13. Understanding the role of this gene and its products is essential in comprehending the importance of messenger RNA synthesis in eukaryotic organisms.
Other names	The DNA-directed RNA polymerase II subunit RPB11-a, also known as hRPB14, POLR2J1, or RPB11, plays an important role in the transcription process. This 13.3 kDa polypeptide is essential for the proper functioning of RNA polymerase II, which is responsible for the synthesis of mRNA in eukaryotic cells. RPB11-a is a small but critical subunit of the RNA polymerase II complex, and mutations in this gene have been associated with several genetic disorders. The function of RPB11-a in RNA polymerase II is to stabilize the whole complex and help it initiate transcription. RPB11-a binds to the RPB1 subunit of the polymerase and facilitates its interaction with the promoter region of genes. This allows the polymerase to initiate transcription and start the process of mRNA synthesis. One interesting aspect of RPB11-a is its multiple isoforms. There are three isoforms of this protein, including RPB11m, RPB11a, and RPB11-HUMAN. Each of these isoforms has a slightly different sequence and may play a unique role in transcription regulation. For example, RPB11a has been shown to interact with specific transcription factors and regulate the expression of certain genes. Overall, RPB11-a is a small but critical component of RNA polymerase II. Its role in stabilizing the complex and facilitating transcription initiation makes it an essential protein for proper gene expression in eukaryotic organisms.
Source	Rabbit
Specificity	The POLR2J Antibody is specifically designed to recognize and measure the natural expression levels of total POLR2J protein in cells.
Species Reactivity	Human;Mouse;Rat
Antigen	POLR2J
Application	The recommended dilution range for using this antibody for Western blotting is 1:500 to 1:2000. For immunohistochemistry, the recommended range is 1:50 to 1:200, while for immunofluorescence or immunocytochemistry, it is 1:100 to 1:500. For ELISA using the peptide, the suggested dilution range is 1:20,000 to 1:40,000. These dilution ratios are based on the original text information and should be used as a guideline for optimal performance of the antibody.
Immunogen	A synthesized peptide derived from human POLR2J.
MW	13kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft (By similarity).
Tissue specificity	Ubiquitously expressed. High expression was found in heart and skeletal muscle.
Celluar localization	Nucleus;
UniPort	P52435