Rabbit Anti-PKN1 Polyclonal Antibody#abs135092
Please note that the price mentioned is for reference purposes only. For detailed pricing information, please get in touch with our sales representative, Vecent. It is important to understand that the price quoted may be subject to change and we recommend speaking with Vecent directly for the...
Description
| Catalog-specification | Delivery time | USD price |
abs135092-100ug | 1-2 Weeks | 301.0 |
abs135092-50ug | 1-2 Weeks | 201.0 |
Please note that the price mentioned is for reference purposes only. For detailed pricing information, please get in touch with our sales representative, Vecent. It is important to understand that the price quoted may be subject to change and we recommend speaking with Vecent directly for the most accurate information.
| Overview | |
catalog | abs135092 |
| Other names | Protease-activated kinase 1, also known as PAK-1, PKN ALPHA, or PRKCL1, is a serine-threonine protein kinase that belongs to the protein kinase C-like family. It is encoded by the PKN1 gene in humans and plays a significant role in cellular processes such as cell motility, cytoskeletal rearrangements, and apoptosis. PKN1 is phosphorylated by upstream signaling molecules such as the Rho family of small GTPases and protein kinase C. It then activates downstream effectors such as LIM domain kinases and myosin light chain kinase, ultimately leading to the regulation of actin cytoskeletal dynamics. PKN1 has been implicated in several diseases such as cancer, Alzheimer's disease, and cardiovascular diseases. Hence, it is a promising target for therapeutic interventions. In summary, PKN1 is a crucial protein kinase that regulates various cellular processes. Its dysregulation has been implicated in various diseases and thus represents a potential therapeutic target. |
| Source | Rabbit |
| Specificity | The PKN1 Antibody is capable of detecting total levels of PKN1 in the body. This detection is based on the endogenous levels of PKN1 present in the body. By utilizing this antibody, it is possible to gain insight into the total levels of PKN1 that are present. |
| Species Reactivity | Human;Mouse;Rat |
| Predictive reaction species | Pig;Bovine;Dog |
| Antigen | PKN1 |
| Application | The recommended dilution for Western Blot is 1:500 to 1:1000, while the dilution range for IF/ICC is 1:100 to 1:500. For ELISA using a peptide, the recommended dilution range is 1:20000 to 1:40000. It is important to follow these dilution recommendations to achieve accurate and reliable results in your experiments. |
| Immunogen | A synthesized peptide. |
| MW | 103 KD |
| Properties | |
Concentration | 1mg/ml |
| purification | SulfoLink™ Coupling Resin was utilized to conduct peptide affinity chromatography for the purification of the antiserum. In this process, the original text information was rearranged while ensuring the generated content remains highly similar to the original text. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | To ensure the longevity of your product, it is recommended that you store it in a temperature-controlled environment of -20 °C for a period of one year. It is important to avoid exposing the product to repeated freeze/thaw cycles, as this can compromise its integrity. So make sure to handle and store it carefully. |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro. |
| Tissue specificity | Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells. |
| Posttranslational modification | Autophosphorylated; preferably on serine. Phosphorylated during mitosis.Activated by limited proteolysis with trypsin.(Microbial infection) In case of infection, polyubiquitinated by the bacterial E3 ubiquitin-protein ligase SspH1, leading to its proteasomal degradation. |
| Celluar localization | Cytosol;Endosome;Nucleus;Plasma Membrane; |
| UniPort | Q16512 |
This product is for research use only, not for use in diagnostic prodecures or in human.
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