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Rabbit Anti-PKD1/2/3 Polyclonal Antibody#abs138388

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Description

Catalog-specification	Delivery time	USD price
abs138388-100ug	1-2 Weeks	301.0
abs138388-50ug	1-2 Weeks	201.0

Overview
catalog	abs138388
Other names	Protein kinases play a crucial role in cell signaling pathways, regulating various physiological processes. One such family is the protein kinase D (PKD) family, which includes PKD1, PKD2, and PKD3. These enzymes are serine/threonine kinases and are involved in the phosphorylation of target proteins. PKD1, also known as PRKD1 or PRKCM, is a member of the PKD family and is often referred to as PKC mu. It plays a role in cell differentiation, proliferation, and survival. PKD2, also known as PRKD2 or PRKCD, is another member of this family and is sometimes referred to as nPKC D2. It has been implicated in the regulation of intracellular processes such as protein transport and apoptosis. The third member of the PKD family is PKD3, also known as PRKD3 or PRKCN. It is often referred to as nPKC nu and is involved in various cellular functions, including cell growth, proliferation, and survival. All three PKD isoforms share a similar domain structure, including an N-terminal regulatory domain and a C-terminal catalytic domain. In summary, the PKD family consists of three closely related protein kinases, PKD1, PKD2, and PKD3, which play important roles in multiple cellular processes. Understanding their functions and regulatory mechanisms may provide insights into various diseases and potential therapeutic targets.
Source	Rabbit
Specificity	The PKD1/2/3 Antibody is effective in identifying the presence of total PKD1/2/3 at their natural levels in the body. This indicates that this antibody is capable of detecting the endogenous levels of total PKD1/2/3.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Sheep;Dog;Bovine;
Antigen	PKD1/2/3
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human PKD1/2/3.
MW	100 kDa
Properties
Concentration	1mg/ml
purification	Through the utilization of SulfoLink™ Coupling Resin, the process of peptide affinity chromatography was employed for the purification of the antiserum. Let's generate a highly similar content by rearranging the given information, while ensuring that the generated content is based on the original text information.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of cell proliferation via MAPK1/3 (ERK1/2) signaling, oxidative stress-induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, signaling downstream of T-cell antigen receptor (TCR) and cytokine production, and plays a role in Golgi membrane trafficking, angiogenesis, secretory granule release and cell adhesion (PubMed:15604256, PubMed:14743217, PubMed:17077180, PubMed:16928771, PubMed:17962809, PubMed:17951978, PubMed:18262756, PubMed:19192391, PubMed:19001381, PubMed:23503467, PubMed:28428613). May potentiate mitogenesis induced by the neuropeptide bombesin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression (By similarity). In response to oxidative stress, is phosphorylated at Tyr-438 and Tyr-717 by ABL1, which leads to the activation of PRKD2 without increasing its catalytic activity, and mediates activation of NF-kappa-B (PubMed:15604256, PubMed:28428613). In response to the activation of the gastrin receptor CCKBR, is phosphorylated at Ser-244 by CSNK1D and CSNK1E, translocates to the nucleus, phosphorylates HDAC7, leading to nuclear export of HDAC7 and inhibition of HDAC7 transcriptional repression of NR4A1/NUR77 (PubMed:17962809). Upon TCR stimulation, is activated independently of ZAP70, translocates from the cytoplasm to the nucleus and is required for interleukin-2 (IL2) promoter up-regulation (PubMed:17077180). During adaptive immune responses, is required in peripheral T-lymphocytes for the production of the effector cytokines IL2 and IFNG after TCR engagement and for optimal induction of antibody responses to antigens (By similarity). In epithelial cells stimulated with lysophosphatidic acid (LPA), is activated through a PKC-dependent pathway and mediates LPA-stimulated interleukin-8 (IL8) secretion via a NF-kappa-B-dependent pathway (PubMed:16928771). During TCR-induced T-cell activation, interacts with and is activated by the tyrosine kinase LCK, which results in the activation of the NFAT transcription factors (PubMed:19192391). In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane and in polarized cells is involved in the transport of proteins from the TGN to the basolateral membrane (PubMed:14743217). Plays an important role in endothelial cell proliferation and migration prior to angiogenesis, partly through modulation of the expression of KDR/VEGFR2 and FGFR1, two key growth factor receptors involved in angiogenesis (PubMed:19001381). In secretory pathway, is required for the release of chromogranin-A (CHGA)-containing secretory granules from the TGN (PubMed:18262756). Downstream of PRKCA, plays important roles in angiotensin-2-induced monocyte adhesion to endothelial cells (PubMed:17951978). Plays a regulatory role in angiogenesis and tumor growth by phosphorylating a downstream mediator CIB1 isoform 2, resulting in vascular endothelial growth factor A (VEGFA) secretion (PubMed:23503467).
Tissue specificity	Widely expressed.
Posttranslational modification	Phosphorylation of Ser-876 correlates with the activation status of the kinase (PubMed:11062248). Ser-706 or/and Ser-710 are probably phosphorylated by PKC (PubMed:12058027, PubMed:28428613). Phosphorylation at Ser-244 by CSNK1D and CSNK1E promotes nuclear localization and substrate targeting (PubMed:17962809). Phosphorylation at Ser-244, Ser-706 and Ser-710 is required for nuclear localization (PubMed:17962809). Phosphorylated at Tyr-438 by ABL1 in response to oxidative stress (PubMed:15604256). Phosphorylated at Tyr-717 by ABL1 specifically in response to oxidative stress; requires prior phosphorylation at Ser-706 or/and Ser-710 (PubMed:28428613).
Celluar localization	Cytosol;Golgi apparatus;Nucleus;Plasma Membrane;
UniPort	Q9BZL6/Q15139/O94806