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Rabbit Anti-PKA CAT Polyclonal Antibody#abs131553

Please consult our seller Vecent for the exact price as the information provided above is for reference purposes only. Western blot analysis of PKA CAT expression in mouse brain cells This product is for research use only, not for use in diagnostic prodecures or in human.

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Description

Catalog-specification	Delivery time	USD price
abs131553-100ug	1-2 Weeks	301.0
abs131553-50ug	1-2 Weeks	201.0

Please consult our seller Vecent for the exact price as the information provided above is for reference purposes only.

Overview
catalog	abs131553
Description	The protein described above is responsible for phosphorylating a large number of substrates located in both the cytoplasm and nucleus. It plays an important role in regulating the abundance of its regulatory subunits by utilizing phosphorylation to target them for proteolysis. In addition, this protein is known to phosphorylate various molecules including CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA, and VASP. One of the outcomes of this phosphorylation is the activation of RORA. Moreover, this protein is required for stimulating adipogenic differentiation in response to glucose and inhibiting osteogenic differentiation from mature osteoblasts.
Other names	Alpha and beta isoforms of cAMP-dependent protein kinase catalytic subunit, also known as PKA C-alpha and PKACB, play crucial roles in cellular signaling. These isoforms, including cAMP-dependent protein kinase catalytic subunit alpha isoform 1 and beta isoform 4ab, are involved in phosphorylation events and regulation of various cellular processes. The cAMP-dependent protein kinase catalytic subunit alpha, commonly referred to as PRKACA or PKACA, is a vital component of PKA-mediated signaling pathways. Its counterpart, cAMP-dependent protein kinase catalytic subunit beta (PRKACB or PKACB), also contributes significantly to cellular signal transduction. Interestingly, the alpha isoform, PKA C-alpha, exhibits distinct features compared to the beta isoform, PKA C-beta. These isoforms, along with PKA C-alpha and PKA C-beta isoforms, contribute to the versatility of PKA-mediated signaling. The protein kinase A catalytic subunit alpha isoform (PRKACAA) and beta isoform (PRKACB) are crucial players in the regulation of cellular processes. Moreover, Protein kinase A catalytic subunit (PKACA) and Protein kinase A catalytic subunit beta (PKACB) act as key regulators of cellular signaling cascades. Additionally, the cAMP-dependent protein kinase catalytic subunit alpha (KAPCA_HUMAN) and beta are essential components in the phosphorylation events. In summary, these various isoforms of cAMP-dependent protein kinase catalytic subunit play vital roles in cellular signal transduction and are essential for maintaining cellular homeostasis.
Source	Rabbit
Specificity	The presence of total PKA CAT can be accurately measured using the PKA CAT Antibody, which is specifically designed to detect endogenous levels of the protein. This antibody provides reliable results and enables researchers to study the overall expression of PKA CAT in various samples.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Xenopus;Sheep;Dog;Bovine;Horse;
Antigen	PKA CAT
Application	The recommended dilutions for using this antibody in Western blotting range from 1:500 to 1:2000. For immunohistochemistry, dilute the antibody at a range of 1:50 to 1:200. When using the antibody in immunofluorescence, the recommended dilution range is between 1:100 and 1:500. For ELISA using a peptide substrate, dilute at a range of 1:20000 to 1:40000. It is important to follow these dilution guidelines for optimal results in your experiments.
Immunogen	A synthesized peptide derived from human PKA CAT.
MW	36 kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca2+, leading to reduced amplitude and increased frequency of store overload-induced Ca2+ release (SOICR) characterized by an increased rate of Ca2+ release and propagation velocity of spontaneous Ca2+ waves, despite reduced wave amplitude and resting cytosolic Ca2+. PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT). Phosphorylates APOBEC3G and AICDA. Isoform 2 phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation. Phosphorylates HSF1; this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock (PubMed:21085490).
Tissue specificity	Isoform 1 is ubiquitous. Isoform 2 is sperm-specific and is enriched in pachytene spermatocytes but is not detected in round spermatids.
Posttranslational modification	Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively.Autophosphorylated. Phosphorylation is enhanced by vitamin K2. Phosphorylated on threonine and serine residues. Phosphorylation on Thr-198 is required for full activity.Phosphorylated at Tyr-331 by activated receptor tyrosine kinases EGFR and PDGFR; this increases catalytic efficienncy.
Celluar localization	Cytoskeleton;Cytosol;Extracellular region or secreted;Mitochondrion;Nucleus;Plasma Membrane;
UniPort	P17612