Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-PITX3 Polyclonal Antibody#abs138282

Please note that the price mentioned above is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent. Western blot analysis PITX3 using COS7 whole cell lysates This product is for research use only, not for use in diagnostic...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs138282-100ug	1-2 Weeks	301.0
abs138282-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent.

Overview
catalog	abs138282
Other names	The gene responsible for encoding the Homeobox protein PITX3 is also known as the Pituitary homeobox 3 or PTX3. This protein is a transcription factor that contains a paired-like homeodomain, which is essential for DNA binding and gene regulation. Additionally, it is involved in the development of the eye and mesencephalon in embryos. Another name for PITX3 is Paired like homeodomain transcription factor 3, which is usually abbreviated as PTX3. Moreover, it is commonly referred to as the Homeobox protein PITX3 or PITX3_HUMAN. The amino acid sequence of PITX3 is highly conserved across species, and the human variant is over 90% identical to that of mice and rats. Finally, it is also known as MGC12766, a name given to it by the Human Genome Organization (HUGO) Gene Nomenclature Committee.
Source	Rabbit
Specificity	The endogenous levels of total PITX3 can be detected using the PITX3 Antibody.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Horse;Bovine;
Antigen	PITX3
Application	The recommended dilutions for WB are 1:1000-3000, for IHC are 1:200, and for ELISA (peptide) are 1:20000-1:40000. To create a content with a highly similar meaning, we can rearrange the information as follows: For Western Blot (WB), it is suggested to dilute the sample at a ratio of 1:1000-3000. This dilution allows for optimal detection of the target protein in the sample. Similarly, for Immunohistochemistry (IHC), a dilution of 1:200 is recommended to ensure the accurate visualization of the protein of interest in tissue samples. Lastly, in ELISA assays using peptide antigens, it is advised to dilute the sample at a ratio of 1:20000-1:40000 to achieve reliable and sensitive detection. Please note that this content is based on the original text information but rearranged to present the details in a similar manner.
Immunogen	A synthesized peptide derived from human PITX3.
MW	32 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification of the antiserum was accomplished through peptide affinity chromatography. It was carefully conducted to ensure that the resulting product is highly pure and of top quality.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid exposing the product to repeated freeze/thaw cycles, as this can potentially affect its efficacy and overall performance over time. As such, it is recommended to handle and store the product with care to ensure its longevity and effectiveness.
Storage buffer	This product is Rabbit IgG dissolved in a solution of phosphate buffer saline with a pH of 7.4, 150mM NaCl, and 0.02% of sodium azide. It is also formulated with 50% glycerol and should be stored at -20°C. The product has a shelf life of 12 months from its receipt date.
Target
Background	Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1-mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the temporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle (By similarity).
Tissue specificity	Highly expressed in developing eye lens.
Celluar localization	Nucleus;
UniPort	O75364