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Rabbit Anti-PADI4 Polyclonal Antibody#abs136205

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Description

Catalog-specification	Delivery time	USD price
abs136205-100ug	1-2 Weeks	301.0
abs136205-50ug	1-2 Weeks	201.0

Overview
catalog	abs136205
Description	This particular gene belongs to a cluster of genes that encode enzymes crucial for altering arginine residues into citrulline residues. Its involvement in the development of granulocytes and macrophages may contribute to the occurrence of inflammation and immune response. Let's explore this gene family's role in detail.
Other names	PADI4 is an enzyme also known as peptidyl arginine deiminase type IV or protein arginine deiminase type IV, which is encoded by the PADI4 gene. It belongs to a family of enzymes that catalyze the conversion of peptidylarginine to citrulline, a process known as citrullination or deimination. PADI4 is widely expressed in human tissues, and it has been implicated in a variety of cellular processes, including gene regulation, apoptosis, and immune function. There is also growing evidence that PADI4 plays a role in the pathogenesis of several autoimmune diseases, such as rheumatoid arthritis, multiple sclerosis, and systemic lupus erythematosus. PADI4 inhibitors may have therapeutic potential in these conditions.
Source	Rabbit
Specificity	The Total PADI4 levels can be detected by the PADI4 Antibody at endogenous levels.
Species Reactivity	Human;Mouse;Rat
Antigen	PADI4
Application	The dilution ranges for WB, IHC, IF/ICC, and ELISA (peptide) are as follows: WB - 1:500-1:2000, IHC - 1:50-1:200, IF/ICC - 1:100-1:500, and ELISA (peptide) - 1:20000-1:40000. These dilution ratios are used to optimize the antibody concentrations for each specific application. It is important to carefully determine the appropriate dilution factor to achieve optimal results in your experiments. Remember to adjust the dilutions based on the specific antibody and sample type being used.
Immunogen	A synthesized peptide derived from human PADI4.
MW	74kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, a peptide affinity chromatography process was employed in the purification of the antiserum. In this way, the specific peptides that were targeted were effectively isolated and removed, resulting in a highly purified antiserum product.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
Tissue specificity	Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.
Posttranslational modification	Autocitrullination at Arg-372 and Arg-374 inactivates the enzyme.
Celluar localization	Cytosol;Nucleus;
UniPort	Q9UM07