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Rabbit Anti-PABPN1 Polyclonal Antibody#abs137307

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Description

Catalog-specification	Delivery time	USD price
abs137307-100ug	1-2 Weeks	301.0
abs137307-50ug	1-2 Weeks	201.0

Overview
catalog	abs137307
Other names	Nuclear poly(A)-binding protein 1, also known as OPMD or PAB2, is a crucial protein involved in RNA processing and gene expression regulation. It plays a pivotal role in controlling mRNA stability, translation efficiency, and mRNA localization within the cell. The protein is encoded by the PABPN1 gene and is commonly referred to as PABP 2, pABP-2, or PABPII. PABP2 is predominantly found within the nucleus of the cell, where it binds to the poly(A) tail of mRNA molecules. By binding to the poly(A) tail, PABP2 helps in stabilizing the mRNA and protecting it from degradation. Additionally, it facilitates the transport of mRNA from the nucleus to the cytoplasm, where the mRNA is eventually translated into protein. Mutations in the PABPN1 gene have been linked to a genetic disorder called oculopharyngeal muscular dystrophy (OPMD). OPMD is characterized by progressive muscle weakness in the muscles of the eyelids and throat, leading to difficulties in swallowing and drooping eyelids. Polyadenylate-binding nuclear protein 1, as PABP2 is sometimes referred to, plays a crucial role in maintaining cellular homeostasis and normal cellular functions. It interacts with various proteins involved in RNA processing, such as RNA polymerase II, poly(A) polymerase, and splicing factors. These interactions contribute to the efficient processing and maturation of mRNA molecules. In summary, PABP2, also known as nuclear poly(A)-binding protein 1 or OPMD, is a vital component of the cellular machinery responsible for mRNA processing and stability. Its role in mRNA regulation highlights its significance in gene expression control and the overall functioning of the cell.
Source	Rabbit
Specificity	The PABPN1 Antibody can recognize the complete amount of endogenous PABPN1 protein. The antibody is designed to identify and target the total amount of PABPN1 present in the sample. This property of the antibody makes it an efficient tool in protein detection experiments for exploring the expression patterns of PABPN1.
Species Reactivity	Human;Mouse
Predictive reaction species	Rabbit;Pig;Dog;Horse;Bovine
Antigen	PABPN1
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human PABPN1.
MW	49 kDa
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography employing SulfoLink™ Coupling Resin was utilized to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	It is recommended to keep the store at -20 °C for a period of one year and prevent the occurrence of repeated freeze/thaw cycles.
Storage buffer	The Rabbit IgG is preserved in phosphate buffered saline at a pH of 7.4. It is supplemented with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. For optimal storage, it is recommended to keep it at a temperature of -20°C. This product maintains its stability for a duration of 12 months starting from the date of receipt.
Target
Background	Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product (By similarity). Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length (By similarity). Increases the affinity of poly(A) polymerase for RNA (By similarity). Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes (PubMed:11371506). Binds to poly(A) and to poly(G) with high affinity (By similarity). May protect the poly(A) tail from degradation (By similarity).
Tissue specificity	Ubiquitous.
Posttranslational modification	Arginine dimethylation is asymmetric and involves PRMT1 and PRMT3. It does not influence the RNA binding properties (By similarity).
Celluar localization	Nucleus;
UniPort	Q86U42