Rabbit Anti-ATF6B Polyclonal Antibody #abs130354

abs130354-50ug

1-2 Weeks

201

abs130354-100ug

1-2 Weeks

301

Other names

ATF6B, also known as activating transcription factor 6 beta, is a protein that functions as a transcription factor. It plays a role in the unfolded protein response, a cellular stress response pathway that is activated when misfolded proteins accumulate in the endoplasmic reticulum. This response helps to restore cellular homeostasis and prevent cell death.
ATF6B is a member of the cAMP response element-binding protein family of transcription factors, which bind to specific DNA sequences in the promoter regions of target genes. It is involved in the regulation of a wide range of genes that play roles in protein folding, transport, and degradation, as well as in the regulation of lipid metabolism and cell survival.
In addition to its role in the unfolded protein response, ATF6B has also been implicated in the regulation of cell growth and differentiation, and in the development of certain diseases, such as cancer and neurodegenerative disorders.
Overall, ATF6B is a key regulator of cellular stress responses and plays important roles in maintaining cellular homeostasis and promoting cell survival. Its precise functions and mechanisms of action are still being elucidated, and further research is needed to fully understand its role in health and disease.

Source

Rabbit

Specificity

ATF6B Antibody detects endogenous levels of ATF6B.

Species Reactivity

Human

Application

For WB, the recommended dilution range is between 1:500 to 1:2000. When performing IF/ICC, the suggested dilution range is between 1:100 to 1:500. For ELISA using peptide, the recommended dilution range is between 1:20000 to 1:40000. It is important to adhere to these dilution ranges in order to obtain accurate and reliable results. Additionally, it is always important to follow the manufacturer's instructions and protocols when performing any experimental procedures.

Immunogen

A synthesized peptide derived from human ATF6B.

Properties

Concentration

1mg/ml

Purification

To obtain purified antiserum, SulfoLink™ Coupling Resin was utilized in a peptide affinity chromatography process. This process facilitated the isolation of the desired antibody molecules, enabling the creation of a highly effective antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure proper storage, it is recommended to keep the product at a temperature of -20 °C for a period of one year. It is important to refrain from exposing the product to repeated freeze and thaw cycles to maintain its integrity and effectiveness.

Storage buffer

The provided content describes the storage condition and stability of Rabbit IgG in phosphate buffered saline. It states that the IgG should be stored at -20 °C in a solution containing phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. The stability period is mentioned to be 12 months from the date of receipt.
To generate a highly similar but rearranged content, one possible version could be:
"Phosphate buffered saline, pH 7.4, containing Rabbit IgG, 150mM NaCl, 0.02% sodium azide, and 50% glycerol is recommended for storage at -20 °C. The solution ensures the stability of Rabbit IgG for up to 12 months from the date of receipt. Please note that the storage conditions should be strictly followed to maintain the functionality of the IgG."

Target

Background

A key transcriptional factor involved in the unfolded protein response (UPR) pathway is responsible for activating UPR target genes that are induced during endoplasmic reticulum (ER) stress. It specifically binds to the DNA sequence 5'-CCAC[GA]-3' within the ER stress response element (ERSE), which is located within the sequence 5'-CCAATN9CCAC[GA]-3', when NF-Y is already bound to the ERSE.

Tissue specificity

Ubiquitous.

Posttranslational modification

The protein in question is N-glycosylated and undergoes proteolysis during the unfolded protein response. This process results in the release of a 60 kDa fragment that contains the transcription factor domain. The cleavage occurs in a sequential manner, with site-1 and site-2 proteases likely responsible for the process.

Celluar localization

Endoplasmic reticulum;Golgi apparatus;Nucleus;

UniPort

Q99941