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Rabbit Anti-PA2G4 Polyclonal Antibody#abs135127

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Description

Catalog-specification	Delivery time	USD price
abs135127-50ug	1-2 Weeks	201.0
abs135127-100ug	1-2 Weeks	301.0

Overview
catalog	abs135127
Other names	This protein, known as PA2G4 or proliferation-associated 2G4, has various aliases such as p38-2G4, Mpp1, Plfap, and hG4-1. It is a 38kDa protein and is also referred to as ErbB3-binding protein 1 or Ebp1 due to its interaction with ErbB-3. PA2G4 is involved in regulating the cell cycle and has been found to be associated with IRES-specific cellular trans-acting factor 45 kDa. It is encoded by the AA672939 gene and is homologous to the cell cycle protein p38 2G4. PA2G4 has also been identified as a proliferation-associated protein and has been implicated in various cellular processes. Other aliases for this protein include MGC81621, MGC94070, and si:dz150i12.2.
Source	Rabbit
Specificity	The PA2G4 Antibody is specifically designed to detect the presence of total PA2G4 at its natural levels within an organism. This highly specific antibody allows for accurate and reliable measurement of PA2G4 levels, aiding in the understanding of its functions and potential role in various biological processes. By detecting endogenous levels of PA2G4, researchers can gain insights into its involvement in cellular mechanisms and potential implications in disease development. Overall, the PA2G4 Antibody provides a valuable tool for studying and elucidating the intricate functions of PA2G4 in a variety of biological contexts.
Species Reactivity	Human;Mouse
Antigen	PA2G4
Application	For Western Blotting, the recommended dilution range is 1:500 to 1:1000. When performing immunofluorescence or immunocytochemistry, the suggested dilution range is 1:100 to 1:500. For ELISA using peptide as the antigen, a dilution range of 1:20000 to 1:40000 is suggested.
Immunogen	A synthesized peptide.
MW	44 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification process of the antiserum involved peptide affinity chromatography. This allowed for highly efficient separation and removal of unwanted impurities, resulting in a high-quality final product.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve its quality, it is recommended to store the product at -20 °C for a period of one year. It is important to avoid subjecting it to multiple freeze and thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site) (By similarity). Regulates cell proliferation, differentiation, and survival. Isoform 1 suppresses apoptosis whereas isoform 2 promotes cell differentiation (By similarity).
Tissue specificity	Isoform 2 is undetectable whereas isoform 1 is strongly expressed in cancer cells (at protein level). Isoform 1 and isoform 2 are widely expressed, including heart, brain, lung, pancreas, skeletal muscle, kidney, placenta and liver.
Posttranslational modification	Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization.In cancer cells, isoform 2 is polyubiquitinated leading to its proteasomal degradation and phosphorylation by PKC/PRKCD enhances polyubiquitination.
Celluar localization	Extracellular region or secreted;Lysosome;Nucleus;
UniPort	Q9UQ80