Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-EIF4G2 Polyclonal Antibody#abs132758

To clarify, the mentioned price is just a reference for you. For specific pricing details, we kindly request you to get in touch with our seller, Vecent. Please note that the generated response will be entirely different from the original text. Western blot analysis of EIF4G2 expression in A431...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs132758-50ug	1-2 Weeks	201.0
abs132758-100ug	1-2 Weeks	301.0

Overview
catalog	abs132758
Description	During mitosis, apoptosis, and viral infection, it seems that this protein is involved in the transition from cap-dependent to IRES-mediated translation. It can be cleaved by certain caspases and viral proteases. Let's rearrange the content to create a similar but rephrased version: The transition from cap-dependent to IRES-mediated translation appears to be facilitated by this protein during mitosis, apoptosis, and viral infection. It undergoes cleavage by specific caspases and viral proteases.
Other names	Death-associated protein 5, also known as DAP-5, is a protein that plays a role in translation initiation. Specifically, it is a subunit of eukaryotic translation initiation factor 4F (eIF-4F), a complex involved in the recruitment of the 40S ribosomal subunit to the mRNA. DAP-5 is also known as eIF-4-gamma 2 or EIF4G2. The protein has been shown to repress translation in certain contexts, such as during stress responses. Additionally, it has been linked to the regulation of apoptosis, the process of programmed cell death. Another name for DAP-5 is Novel APOBEC-1 target 1, or Nat1, which refers to its identification as a protein that interacts with the RNA-editing enzyme APOBEC-1. Despite its multiple names and functions, DAP-5 is mostly recognized for its involvement in translation initiation and its potential role in cell death regulation.
Source	Rabbit
Specificity	The capability of the EIF4G2 antibody to identify total EIF4G2 expressed within an organism is quite remarkable. Utilizing this antibody, one can detect native levels of EIF4G2 without any external manipulation or interference. This implies that the antibody enables researchers to examine the presence and quantity of EIF4G2 within a biological system accurately. Therefore, the EIF4G2 antibody provides a valuable tool for investigating the functions and mechanisms involving EIF4G2 in various biological contexts.
Species Reactivity	Human;Mouse;Rat
Antigen	EIF4G2
Application	The dilution ranges for WB are 1:500-1:2000, for IF/ICC are 1:100-1:500, and for ELISA(peptide) are 1:20000-1:40000. These dilution ranges indicate the recommended concentrations of primary antibodies to be used in various experimental techniques. For Western Blot (WB), it is suggested to dilute the primary antibody at a ratio of 1:500 to 1:2000 with the appropriate buffer solution. This range ensures optimal binding of the primary antibody to the target protein on the blot. Similarly, for Immunofluorescence (IF) or Immunocytochemistry (ICC), the primary antibody should be diluted at a ratio of 1:100 to 1:500 to achieve reliable and specific staining of the target antigen. Finally, for ELISA assays using peptide antigens, it is recommended to dilute the primary antibody at a range of 1:20000 to 1:40000 in order to obtain accurate and sensitive detection of the peptide. These dilution ranges have been optimized and tested to provide optimal performance and signal-to-noise ratio in these experimental techniques.
Immunogen	A synthesized peptide.
MW	102 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent a purification process via peptide affinity chromatography. This resulted in a highly refined product, free from impurities.
Clonality	Polyclonal Antibody
Stability & Storage	The recommended storage condition for a one-year duration is at -20 °C. It is important to avoid subjecting the stored item to multiple freeze/thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Target
Background	Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.
Tissue specificity	Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney.
Posttranslational modification	Phosphorylation; hyperphosphorylated during mitosis.
Celluar localization	Cytosol;
UniPort	P78344