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Rabbit Anti-Ob Polyclonal Antibody#abs138103

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Description

Catalog-specification	Delivery time	USD price
abs138103-100ug	1-2 Weeks	301.0
abs138103-50ug	1-2 Weeks	201.0

Overview
catalog	abs138103
Other names	LEP_HUMAN, also known as FLJ94114, is a protein that is important in regulating body weight and energy metabolism. It is the human homolog of the murine obesity protein, Leptin. Leptin plays a crucial role in signaling to the brain to suppress appetite and increase energy expenditure. This obesity factor is an essential molecule in understanding and addressing obesity-related disorders. Leptin, also called the Obese protein, was initially discovered in mice and later found to have a counterpart in humans. It is a hormone produced mainly by adipose tissue, but also by several other organs, including the stomach, placenta, and skeletal muscle. Its primary function is to regulate energy balance by inhibiting hunger and promoting feelings of satiety. Leptin acts by binding to receptors in the hypothalamus, a region of the brain involved in controlling appetite and metabolism. Upon binding, it signals the brain to reduce food intake and increase energy expenditure, effectively regulating body weight. Deficiencies in leptin or its receptor can lead to severe obesity, as seen in mice lacking the Ob gene. Understanding the mechanisms behind leptin signaling has been instrumental in uncovering insights into the complex regulation of energy balance and obesity. While the murine model initially provided valuable evidence, the discovery of LEP_HUMAN in humans further broadened our understanding of this crucial hormone's role. Overall, Leptin and its human homolog, LEP_HUMAN, are vital proteins involved in maintaining energy homeostasis and regulating body weight. Their study has provided significant insights into the pathophysiology of obesity and potential therapeutic targets for its treatment.
Source	Rabbit
Specificity	Ob Antibody detects endogenous levels of total Ob.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Sheep;Bovine;Horse;Chicken;
Antigen	Ob
Application	According to the original text information, the dilutions for Western blot (WB) can range from 1:1000 to 1:3000. For immunohistochemistry (IHC), the recommended dilution is 1:200. As for ELISA with peptide, the dilutions can vary from 1:20000 to 1:40000. Please note that this would be a highly similar content rearranged version of the original text, rather than a completely different approach to generating content by the language model.
Immunogen	A synthesized peptide derived from human Ob.
MW	18 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, we carried out peptide affinity chromatography to purify the antiserum. The process ensured that the resultant antiserum was of high quality and highly effective in its intended use.
Clonality	Polyclonal Antibody
Stability & Storage	For a period of one year, the storage temperature should be maintained at -20 °C. It is important to prevent the product from undergoing repeated freeze/thaw cycles.
Storage buffer	The storage conditions for Rabbit IgG are as follows: it is suspended in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C and is stable for a period of 12 months starting from the date of receipt.
Target
Background	Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways (PubMed:17344214, PubMed:15899045, PubMed:19688109). In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity (By similarity) (PubMed:8589726, PubMed:11460888, PubMed:19688109, PubMed:24340098, PubMed:25060689). In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides (By similarity). In addition to its known satiety inducing effect, has a modulatory role in nutrient absorption. In the intestine, reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38, PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption (PubMed:24340098). Acts as a growth factor on certain tissues, through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1, via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1 pathways (By similarity) (PubMed:17344214). May also play an apoptotic role via JAK2-STAT3 pathway and up-regulation of BIRC5 expression (PubMed:18242580). Pro-angiogenic, has mitogenic activity on vascular endothelial cells and plays a role in matrix remodeling by regulating the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) (PubMed:11460888). In innate immunity, modulates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic ability of NK cells (PubMed:12504075). Plays a pro-inflammatory role, in synergy with IL1B, by inducing NOS2 wich promotes the production of IL6, IL8 and Prostaglandin E2, through a signaling pathway that involves JAK2, PI3K, MAP2K1/MEK1 and MAPK14/p38 (PubMed:15899045, PubMed:19688109). In adaptive immunity, promotes the switch of memory T-cells towards T helper-1 cell immune responses (By similarity). Increases CD4+CD25- T-cell proliferation and reduces autophagy during TCR (T-cell receptor) stimulation, through MTOR signaling pathway activation and BCL2 up-regulation (PubMed:25060689).
Tissue specificity	Adipose tissue is the main source of leptin it is also produced by other peripheral tissues such as the skeletal muscle (PubMed:7789654, PubMed:16052473, PubMed:12448771). Expressed by intercalated and striated tracts of submandibular and parotid salivary gland intralobular ducts (PubMed:12448771). Detected by fundic epithelium of the gastric mucosa (PubMed:10896907). Secreted into blood and gastric juice (PubMed:10896907).
Celluar localization	Extracellular region or secreted;
UniPort	P41159