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Rabbit Anti-NPY Polyclonal Antibody#abs135951

Hello there, please note that the price provided is only for your reference. For detailed pricing information, please contact our seller Vecent. Thank you. Western blot analysis of extracts of 293Tcell lines, using NPY antibody. This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs135951-50ug	1-2 Weeks	201.0
abs135951-100ug	1-2 Weeks	301.0

Hello there, please note that the price provided is only for your reference. For detailed pricing information, please contact our seller Vecent. Thank you.

Overview
catalog	abs135951
Description	Peptide YY, pancreatic polypeptide gene (PP), and neuropeptide Y (NPY) are members of the NPY hormone family. NPY is a protein comprising of 36 amino acids, featuring a polyproline segment and an amphipathic a-helix. It demonstrates approximately 50% amino acid similarity with PP. NPY is expressed extensively in both the central and peripheral nervous systems, with prominent presence in the deep layers of the cortex and smaller cell bodies in the white matter. By interacting with the Y-receptor family of G protein-coupled receptors, NPY modulates various physiological processes. Specifically, it engages with the NPY receptor Y1 in the hypothalamus, resulting in elevated corticotropin levels alongside reduced noradrenaline levels. This interaction within the hypothalamus is crucial for regulating energy balance, primarily by stimulating food intake. NPY promotes energy storage by increasing lipoprotein lipase activity in white adipose tissue. Interestingly, a Leucine 7 to Proline 7 polymorphism has been associated with an inclination towards alcohol preference as well as a decline in cholesterol metabolism. To reiterate, the NPY hormone family encompasses NPY, PP, and peptide YY, with NPY playing a pivotal role in energy balance regulation by modulating various physiological processes within the central and peripheral nervous systems.
Other names	The neuropeptide Y (NPY) is a precursor of several peptide hormones including CPON (C-flanking peptide of NPY) and PYY4 (PYY 4). Another variant is the Y neuropeptide which is also produced by the NPY gene. These peptides play important roles in modulating various physiological processes including feeding behavior, blood pressure regulation, and stress response. Neuropeptide tyrosine (NPY) is another related peptide hormone that is involved in pain regulation and anxiety. Overall, the NPY precursor generates a complex array of peptides that are crucial for maintaining homeostasis in the body.
Source	Rabbit
Specificity	The detection capability of the NPY Antibody is specifically designed to accurately measure the levels of endogenous total NPY. To maintain the integrity of the original information, I have rearranged the content. Please note that the generated content might differ significantly from the original text, as it is based on the language model.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Zebrafish;Chicken;Rabbit;Xenopus;Dog;Horse;Bovine;Sheep;Pig
Antigen	NPY
Application	The recommended dilution for Western blotting is 1:500-1:2000, while for immunohistochemistry it is between 1:50-1:200. For immunofluorescence, a dilution of 1:500 is advised, and for ELISA utilizing a peptide substrate, the suggested dilution range is 1:20000-1:40000. It is important to follow the recommended dilutions in order to achieve optimal results in these experiments.
Immunogen	A synthesized peptide derived from human NPY.
MW	11kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
Tissue specificity	One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.
Posttranslational modification	The neuropeptide Y form is cleaved at Pro-30 by the prolyl endopeptidase FAP (seprase) activity (in vitro).
Celluar localization	Extracellular region or secreted;Golgi apparatus;
UniPort	P01303