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Rabbit Anti-COL5A1 Polyclonal Antibody#abs135954

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Description

Catalog-specification	Delivery time	USD price
abs135954-100ug	1-2 Weeks	301.0
abs135954-50ug	1-2 Weeks	201.0

Overview
catalog	abs135954
Other names	The protein known as alpha 1 type V collagen is encoded by the COL5A1 gene. It is also referred to as collagen alpha-1(V) chain or simply Collagen V.
Source	Rabbit
Specificity	The endogenous levels of total COL5A1 can be detected using the COL5A1 Antibody. This antibody is designed to specifically target and detect the levels of COL5A1 found within the body. By utilizing this antibody, researchers are able to analyze and measure the levels of COL5A1 present in various biological samples. This information is valuable in understanding the expression and regulation of COL5A1 and its potential implications in different physiological and pathological processes.
Species Reactivity	Human;Mouse;Rat
Antigen	COL5A1
Application	The dilution ranges for WB, IHC, and IF/ICC are as follows: WB can be diluted at a ratio of 1:500 to 1:2000, IHC at a ratio of 1:50 to 1:200, and IF/ICC at a ratio of 1:100 to 1:500. For ELISA using a peptide, the recommended dilution is between 1:20000 to 1:40000. Please note that these dilutions are based on the original information provided.
Immunogen	A synthetic peptideof human COL5A1.
MW	184kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed in the peptide affinity chromatography process to purify the antiserum. The resulting product was highly refined and effective.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure the preservation of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this could negatively impact its quality and effectiveness. Take caution to follow these storage instructions accurately.
Storage buffer	The storage conditions for Rabbit IgG are as follows: it should be kept in phosphate buffered saline at a pH of 7.4, with a NaCl concentration of 150mM. To prevent bacterial growth, 0.02% sodium azide has been added to the solution. Additionally, a 50% glycerol solution has been included in order to maintain the integrity of the antibody. It is important to store this product at a temperature of -20 °C. The stability of Rabbit IgG is guaranteed for a period of 12 months from the date of its receipt.
Target
Background	Type V collagen, belonging to the group I collagen family, is a collagen type that primarily forms fibrils. It is found in various connective tissues throughout the body, although its presence is limited. Interestingly, type V collagen exhibits binding capabilities to a wide range of substances such as DNA, heparan sulfate, thrombospondin, heparin, and insulin. This unique characteristic allows it to interact with multiple molecules, contributing to its versatile role in various biological processes.
Posttranslational modification	Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.Sulfated on 40% of tyrosines.
Celluar localization	Endoplasmic reticulum;Extracellular region or secreted;
UniPort	P20908