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Rabbit Anti-NOX4 Polyclonal Antibody#abs136444

Kindly note that the price mentioned above is for your reference only. For detailed pricing information, please reach out to our seller, Vecent. Western blot analysis of extracts of variouscell lines, using NOX4 antibody. This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs136444-100ug	1-2 Weeks	301.0
abs136444-50ug	1-2 Weeks	201.0

Kindly note that the price mentioned above is for your reference only. For detailed pricing information, please reach out to our seller, Vecent.

Overview
catalog	abs136444
Description	NOX4, also known as NADPH oxidase 4, is an oxidase found in phagocytes, similar to the one responsible for generating large amounts of reactive oxygen species (ROS) in neutrophil granulocytes. This leads to antimicrobial activity. Interestingly, NOX4 has also been suggested to act as an oxygen sensor in the kidney, specifically in the renal cortex, to regulate the production of erythropoietin. It is worth noting that NOX4 exhibits a variety of isoforms, with different molecular weights. These isoforms include 7KDa, 26-32KDa, 58-67KDa, and 75-80KDa. Researchers have identified these isoforms in a study (PMID:11728818). This particular antibody is highly specific to NOX4, making it useful in studies and experiments focused on this protein.
Other names	Oxidase-1 Kidney; Oxidase 1 Kidney; NADPH Oxidase Producing Superoxide Kidney; NADPH Oxidase-1 Producing Superoxide Kidney; KOX 1; KOX; Kox-1; KOX1; NADPH; NADPH Oxidase 4; Nox4; NOX4_HUMAN; Renal Oxidase NAD(P)H; Renal NAD(P)H Oxidase; RENOX;. Rearranged "Kidney oxidase 1" related information: - Kidney oxidase-1, also known as KOX 1 or Kox-1, is an enzyme found in the kidney. - It is responsible for producing superoxide by using NADPH as a cofactor. - The enzyme, also referred to as NADPH oxidase 4 or Nox4, plays a crucial role in renal NAD(P)H oxidation. - Renal NAD(P)H oxidase, or RENOX, is another name for this kidney oxidase. - NOX4_HUMAN is the gene encoding this oxidase in humans.
Source	Rabbit
Specificity	The NOX4 Antibody is capable of detecting total NOX4 levels that are present within the body naturally. To create a similar content, the original information should be rearranged in order to remain true to the text's details.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Sheep;Bovine;Horse;Rabbit
Antigen	NOX4
Application	The dilution range for WB is 1:500-1:2000, for IHC is 1:50-1:200, and for ELISA using a peptide is 1:20000-1:40000.
Immunogen	A synthesized peptide derived from human NOX4.
MW	67kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, a peptide affinity chromatography process was employed to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity.
Tissue specificity	Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells.
Posttranslational modification	Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function.
Celluar localization	Cytoskeleton;Endoplasmic reticulum;Mitochondrion;Nucleus;Plasma Membrane;
UniPort	Q9NPH5