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Rabbit Anti-NFATC2 Polyclonal Antibody#abs136709

Please note that the price mentioned above is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent. Western blot analysis of extracts of mouse fetal brain cellline, using NFATC2antibody. This product is for research use only, not for use in...

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Description

Catalog-specification	Delivery time	USD price
abs136709-100ug	1-2 Weeks	301.0
abs136709-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent.

Overview
catalog	abs136709
Description	This particular gene is classified as a member of the nuclear factor of activated T cells (NFAT) family, and it encodes a protein that is capable of binding to DNA. This protein contains two distinct regions, namely the REL-homology region (RHR) and the NFAT-homology region (NHR), which allows it to play a crucial role in the process of immune response. Initially, this protein is located in the cytosol but only moves into the nucleus once the T cell receptor (TCR) has been activated. At this point, it becomes a key component of the nuclear factors of activated T cells transcription complex, working to induce gene transcription during the immune response. Numerous alternate transcriptional splice variants, which produce differing isoforms of the protein, have been identified, further emphasizing the importance and complexity of its role in immune activation.
Other names	NFATc2, also known as cytoplasmic 2 or KIAA0611, is a transcription factor that plays an important role in the regulation of gene expression in T cells. It is a member of the nuclear factor of activated T cells (NFAT) family, specifically the NFATp and NF-ATc2 subfamilies. NFATc2 is involved in the calcineurin-dependent signaling pathway, where it translocates from the cytoplasm to the nucleus upon activation. NFATc2, also referred to as NFAC2_HUMAN or NFAT1, is a pre-existing subunit of the NFAT transcription complex. As a pre-existing component, it is already present in the nucleus and can rapidly respond to activation signals. NFATc2 functions as a transcription factor, binding to specific DNA sequences and regulating the expression of target genes involved in T-cell activation and immune response. The T-cell transcription factor NFATc2, also known as NFATp, is an essential mediator of T-cell activation and differentiation. It plays a crucial role in the immune response by regulating the expression of genes involved in the activation and proliferation of T cells. NFATc2 is an important component of the NFAT transcription complex, which is essential for the activation of T-cell-specific genes. In summary, NFATc2 is a cytoplasmic calcineurin-dependent transcription factor of the NFAT family. It serves as a pre-existing subunit of the NFAT transcription complex and plays a critical role in T-cell activation and immune response. NFATc2 is involved in gene expression regulation and is an important factor for T-cell-specific gene activation.
Source	Rabbit
Specificity	The total NFATC2 levels can be detected by using the NFATC2 Antibody, which is designed to specifically recognize the endogenous levels of NFATC2. To provide a different perspective, one can say that the NFATC2 Antibody has the ability to effectively identify and quantify the overall expression of NFATC2 proteins within a sample.
Species Reactivity	Human;Mouse;Rat
Antigen	NFATC2
Application	WB 1:500-1:1000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthetic peptide of humanNFATC2.
MW	98kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway.
Tissue specificity	Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Isoform 1 is highly expressed in the small intestine, heart, testis, prostate, thymus, placenta and thyroid. Isoform 3 is highly expressed in stomach, uterus, placenta, trachea and thyroid.
Posttranslational modification	In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.Ubiquitinated in endothelial cells by RNF213 downstream of the non-canonical Wnt signaling pathway, leading to its degradation by the proteasome.
Celluar localization	Cytosol;Nucleus;
UniPort	Q13469