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Rabbit Anti-NCBP1 Polyclonal Antibody#abs133548

Description

Catalog-specification	Delivery time	USD price
abs133548-100ug	1-2 Weeks	301.0
abs133548-50ug	1-2 Weeks	201.0

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Overview
catalog	abs133548
Other names	The 80 kDa nuclear cap-binding protein, also known as CBP80 or Sto1, is a crucial component of the pre-mRNA processing machinery. It is encoded by the NCBP1 gene and is also referred to as NCBP or NCBP 80 kDa subunit. This protein binds to the 5' cap structure of nascent mRNA molecules, playing a vital role in mRNA export, stability, and translation initiation. The NCBP1 gene is located on chromosome 1 and codes for a 80 kDa protein with a conserved RNA recognition motif (RRM) and a zinc finger domain. The protein is highly conserved across species, reflecting its key role in gene expression regulation. The NCBP is involved in multiple stages of pre-mRNA processing, including capping, splicing, and polyadenylation. It interacts with other proteins involved in these processes, such as the cap methyltransferase and the exon junction complex. It also participates in the quality control of mRNA molecules, mediating their degradation or sequestration in P-bodies under certain conditions. Mutations in the NCBP1 gene have been associated with various disorders, including intellectual disability and a form of lung cancer. Research on the NCBP1 protein is ongoing, and its precise mechanisms of action in pre-mRNA processing and gene expression regulation are still being elucidated.
Source	Rabbit
Specificity	The NCBP1 Antibody is specifically designed to detect total NCBP1 at the endogenous levels. This means that it can accurately measure the amount of NCBP1 present in a sample without interference from other proteins or contaminants. The antibody is highly sensitive and reliable in its detection, allowing for precise analysis of NCBP1 levels in a range of biological systems. If you need to measure NCBP1 levels in your research, this antibody is an excellent choice for accurate and reproducible results.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Sheep;Dog;Bovine;
Antigen	NCBP1
Application	The recommended dilution for Western blotting is 1:500 to 1:1000, while for immunofluorescence and immunocytochemistry the suggested dilution range is 1:100 to 1:500. In the case of ELISA using peptide as the antigen, the optimal dilution range is 1:20000 to 1:40000.
Immunogen	A synthesized peptide.
MW	80 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification of the antiserum was carried out through peptide affinity chromatography. To ensure the highest possible quality and purity of the final product, this method was employed. The resulting antiserum is of excellent quality and ideal for a wide range of applications.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the optimal condition of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid exposing the product to repeated cycles of freezing and thawing, as this can compromise its quality and efficacy. By following these storage instructions, you can ensure that the product remains effective and stable for extended periods of time. So be sure to store it in a cool and dry place, away from direct sunlight and other sources of heat and moisture.
Storage buffer	This solution contains Rabbit IgG dissolved in phosphate buffered saline with a pH of 7.4. It also contains 150mM NaCl, 0.02% sodium azide and 50% glycerol. This solution should be stored at a temperature of -20°C and can remain stable for 12 months from the date of receipt.
Target
Background	Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure. Associates with NCBP3 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export and is particularly important in cellular stress situations such as virus infections. The conventional CBC with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus whereas the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role only in mRNA export. NCBP1/CBP80 is required for cell growth and viability (PubMed:26382858).
Celluar localization	Cytosol;Mitochondrion;Nucleus;
UniPort	Q09161