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Phospho-TGFBR1 (Thr204) Rabbit Polyclonal Antibody#abs140285

Please consult our seller Vecent for the specific price details as the mentioned price is only for your reference. Western blot analysis TGFBR1 (Phospho-Thr204) using HepG2 whole cell lysates This product is for research use only, not for use in diagnostic prodecures or in human.

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Description

Catalog-specification	Delivery time	USD price
abs140285-100ug	1-2 Weeks	301.0
abs140285-50ug	1-2 Weeks	201.0

Please consult our seller Vecent for the specific price details as the mentioned price is only for your reference.

Overview
catalog	abs140285
Other names	53kDaAII;53kDAII;53kDaIIA;53kDIIA;53kDaIIA;53kDIIA;53kDaA;53kDA;Activin5;Activin5; ACVRLK 4; ACVRLK4; ALK 5; ALK-5; ALK5; LDS1A; LDS2A; MSSE;/R4;/R4;SKR 4;SKR4;TbetaR I;TbetaR-I;TGF beta1;TGF betaI;TGF betaI;TGF-betaI;TGF-beta1;TGF-betaI; TGFBR 1; TGFBR1; TGFBR1; TGFR 1; TGFR-1; TGFR1; TGFR1_HUMAN;β1;βI(AII,53kD);βI;β1;βI(AII,53kD);βI;。，。
Source	Rabbit
Specificity	The TGFBR1 (Phospho-Thr204) Antibody is designed to specifically recognize and bind to the TGFBR1 protein. It has been validated to detect the protein only when it is phosphorylated at the Thr204 site. This means that the antibody is able to specifically identify and target the activated, phosphorylated form of TGFBR1 in samples. It is a valuable tool for researchers studying the role and signaling of TGFBR1 in various biological processes.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Xenopus;Rabbit;Pig;Dog;Sheep;Bovine;
Antigen	TGFBR1
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	TGFBR1（Phospho-Thr204）。，。
MW	56 kDa.
Properties
Concentration	1mg/ml
purification	The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Transmembrane serine/threonine kinase forming with the TGF-beta type II serine/threonine kinase receptor, TGFBR2, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. For instance, TGFBR1 induces TRAF6 autoubiquitination which in turn results in MAP3K7 ubiquitination and activation to trigger apoptosis. Also regulates epithelial to mesenchymal transition through a SMAD-independent signaling pathway through PARD6A phosphorylation and activation.
Tissue specificity	Found in all tissues examined, most abundant in placenta and least abundant in brain and heart. Expressed in a variety of cancer cell lines (PubMed:25893292).
Posttranslational modification	Phosphorylated at basal levels in the absence of ligand. Activated upon phosphorylation by TGFBR2, mainly in the GS domain. Phosphorylation in the GS domain abrogates FKBP1A-binding.N-Glycosylated.Ubiquitinated; undergoes ubiquitination catalyzed by several E3 ubiquitin ligases including SMURF1, SMURF2 and NEDD4L2. Results in the proteasomal and/or lysosomal degradation of the receptor thereby negatively regulating its activity. Deubiquitinated by USP15, leading to stabilization of the protein and enhanced TGF-beta signal. Its ubiquitination and proteasome-mediated degradation is negatively regulated by SDCBP (PubMed:25893292).
Celluar localization	Endosome;Plasma Membrane;
UniPort	P36897