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Rabbit Anti-FUBP1 Polyclonal Antibody#abs133261

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Description

Catalog-specification	Delivery time	USD price
abs133261-100ug	1-2 Weeks	301.0
abs133261-50ug	1-2 Weeks	201.0

Overview
catalog	abs133261
Other names	Far upstream element binding protein 1 (FUBP1), also known as FUSE binding protein 1, is a DNA helicase V that plays a crucial role in regulating gene expression. It interacts with the far upstream element (FUSE) in the promoter region of certain genes, including FUBP4. FUBP1 is a highly conserved protein in different species, and it is essential for proper gene transcription and cellular processes. FUBP1 is involved in various cellular functions, such as DNA replication, repair, and recombination. Its binding to the FUSE element facilitates the formation of transcription complexes and promotes the activation of target genes. Additionally, FUBP1 has been implicated in the regulation of telomerase, which plays a vital role in maintaining chromosome stability and telomere length. In humans, FUBP1 has been extensively studied due to its association with numerous diseases, including cancer. Altered expression or mutations in FUBP1 have been reported in several cancer types, suggesting its potential as a prognostic marker and therapeutic target. Another member of the FUBP family is FUBP4, which shares similar functions but exhibits distinct expression patterns and regulatory roles. FUBP4 plays a role in mRNA processing and transport, and its dysregulation has been implicated in cancer development and progression. In summary, FUBP1 and FUBP4 are crucial proteins involved in the regulation of gene expression and various cellular processes. Understanding their functions and dysregulation mechanisms can provide valuable insights into disease pathogenesis and potential therapeutic strategies.
Source	Rabbit
Specificity	The detection of total FUBP1 is enabled by the FUBP1 Antibody, which specifically identifies endogenous levels of the protein. To produce similar content by rearranging the given information: "Total FUBP1 can be effectively measured using the FUBP1 Antibody, which is capable of detecting endogenous levels of the protein."
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse;
Antigen	FUBP1
Application	Here are several variations of the provided text: - The recommended dilution for Western blotting is 1:1000 to 1:3000, while for immunohistochemistry it is 1:200. For ICC/IF and ELISA using a peptide substrate, the suggested dilution range is 1:20000 to 1:40000. - Depending on the application, different dilutions are advised for this antibody. For Western blotting, it is suggested to use a dilution range of 1:1000 to 1:3000, while for immunohistochemistry a dilution of 1:200 is recommended. The dilution range for ICC/IF and ELISA using a peptide substrate is broader, from 1:20000 to 1:40000. - To achieve the best results with this antibody, it is advised to use the recommended dilutions depending on the application. For Western blotting, the suggested range is 1:1000 to 1:3000, while for immunohistochemistry a dilution of 1:200 is recommended. If using ICC/IF or ELISA with a peptide substrate, a wider dilution range of 1:20000 to 1:40000 is optimal. - Proper dilution of this antibody is crucial for obtaining precise results. For Western blotting, it is recommended to use a dilution range of 1:1000 to 1:3000, while for immunohistochemistry a dilution of 1:200 is advised. If performing ICC/IF or ELISA with a peptide substrate, the dilution range should be kept between 1:20000 and 1:40000. - For optimal results with this antibody, it is important to use the recommended dilution range depending on the experiment. For Western blotting, the suggested range is 1:1000 to 1:3000, while for immunohistochemistry a dilution of 1:200 is preferred. The dilution range for ICC/IF and ELISA assays with a peptide substrate should stay within 1:20000 and 1:40000.
Immunogen	A synthesized peptide derived from human FUBP1.
MW	74kDa
Properties
Concentration	1mg/ml
purification	We utilized SulfoLink™ Coupling Resin for peptide affinity chromatography during the purification of the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the product's quality, it is recommended to keep it stored at a temperature of -20 °C for a duration of one year. It is crucial to avoid subjecting the product to multiple freeze/thaw cycles to avoid compromising its integrity.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.
Posttranslational modification	Ubiquitinated. This targets the protein for proteasome-mediated degradation.
Celluar localization	Nucleus;
UniPort	Q96AE4