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Rabbit Anti-MAOB Polyclonal Antibody#abs135984

Please note that the price provided is for reference purposes only. For the actual pricing details, we kindly request that you contact our seller, Vecent. Thank you. Western blot analysis of extracts of variouscell lines, using MAOB antibody. This product is for research use only, not for use in...

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Description

Catalog-specification	Delivery time	USD price
abs135984-100ug	1-2 Weeks	301.0
abs135984-50ug	1-2 Weeks	201.0

Please note that the price provided is for reference purposes only. For the actual pricing details, we kindly request that you contact our seller, Vecent. Thank you.

Overview
catalog	abs135984
Description	The MAOB gene encodes a protein weighing 58.8 kD and comprised of 520 amino acids. This protein is highly similar to MAOA, exhibiting 70% amino acid identity, and both enzymes are found in the outer mitochondrial membrane of the central nervous system and peripheral tissues. Examination of purified MAOA and MAOB from human placenta and liver, respectively, reveals a size difference of 2 kDa in the larger A form and a single potential N-glycosylation site in each protein, situated at different key locations. This highly specific antibody is targeted towards MAOB and is not intended for MAOA analysis.
Other names	Human monoamine oxidase B (MAO-B), also known as amine oxidase [flavin-containing] B or tyramine oxidase, is a pivotal enzyme involved in the oxidative deamination of biogenic amines. It plays a crucial role in neurotransmitter metabolism and is found in various tissues including the brain and platelets. MAO-B belongs to the family of amine oxidases containing flavin cofactors. Another isoform of this enzyme, monoamine oxidase A (MAO-A), is also present in the human body. However, for this discussion, the focus will be on MAO-B. MAO-B catalyzes the oxidation of a diverse range of monoamine neurotransmitters, including dopamine, serotonin, and noradrenaline. This enzymatic activity results in the formation of their corresponding aldehydes and hydrogen peroxide, which are subsequently metabolized further by other enzymes in the cellular antioxidant defense systems. The expression of MAO-B is particularly high in astrocytes, a type of glial cell in the brain. This localization suggests its involvement in regulating the concentration of neurotransmitters in the synaptic cleft. By breaking down excess monoamines, MAO-B helps maintain neurotransmitter homeostasis and prevents the accumulation of potentially toxic metabolites. In addition to its role in neurotransmitter metabolism, MAO-B has also been implicated in various neurological disorders. For instance, studies have shown a correlation between increased MAO-B activity and Parkinson's disease. Inhibitors of MAO-B have been developed as therapeutic agents for the treatment of this neurodegenerative disorder. In conclusion, human monoamine oxidase B is an important enzyme involved in the metabolism of monoamine neurotransmitters. Its catalytic activity and tissue distribution make it a key player in maintaining neurological function and neurotransmitter balance. Further research on MAO-B may provide insights into the pathogenesis of neurological disorders and aid the development of novel therapeutic strategies.
Source	Rabbit
Specificity	The MAOB Antibody is designed to recognize and measure the levels of total MAOB protein present in biological samples. This antibody specifically targets the endogenous levels of MAOB, allowing for accurate detection and quantification. Whether used for research purposes or clinical applications, the MAOB Antibody offers a reliable tool to study the expression and activity of this important protein.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Bovine
Antigen	MAOB
Application	The recommended dilutions for using this antibody in various applications are as follows: for Western blotting, dilute at 1:500 to 1:2000; for immunohistochemistry, dilute at 1:50 to 1:200; for immunofluorescence, dilute at 1:100 to 500; for ELISA with peptide, dilute at 1:20000 to 1:40000. It is important to note the appropriate dilution for each application to achieve optimal results.
Immunogen	A synthesized peptide derived from human MAOB.
MW	59kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine.
Celluar localization	Extracellular region or secreted;Mitochondrion;
UniPort	P27338