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Rabbit Anti-IL-6R Polyclonal Antibody#abs135986

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Description

Catalog-specification	Delivery time	USD price
abs135986-100ug	1-2 Weeks	301.0
abs135986-50ug	1-2 Weeks	201.0

Overview
catalog	abs135986
Description	IL-6, also known as interleukin-6, is a versatile cytokine that is produced by various cells in response to infection, trauma, and immune challenges (PMID: 8274730). Its signaling activity relies on a receptor complex composed of two different membrane-bound glycoproteins: the IL-6-binding subunit (IL-6R or CD126 or gp80) and the signal-transducing element (gp130 or CD130), weighing 80-kDa and 130-kDa, respectively. Upon binding of IL-6 to the IL-6R on the cell membrane, the IL-6/IL-6R complex associates with gp130, initiating receptor activation (PMID: 9716487). The distribution of IL-6R and gp130 is characterized by variation. While gp130 is expressed in nearly all organs, IL-6R expression is primarily observed in hepatocytes and specific leukocyte subtypes such as monocytes, neutrophils, T cells, and B cells (PMID: 11149892). Interestingly, the soluble form of IL-6R has also been detected in human serum and urine. These findings highlight the involvement of IL-6 in various physiological processes and its role as a communicator in immune responses and inflammatory conditions.
Other names	The IL-6 receptor alpha chain, also known as CD126 or CD126 antigen, is a transmembrane glycoprotein that plays a crucial role in mediating the effects of interleukin-6 (IL-6). It is encoded by the IL6R gene and is involved in various biological processes, including inflammation, immune response, and hematopoiesis. The IL-6 receptor alpha is also responsible for forming a signaling complex with other receptor chains, such as the IL-6 receptor beta, to initiate downstream signaling cascades. Understanding the function and regulation of IL-6 receptor alpha can provide valuable insights into the pathogenesis of inflammatory diseases and may lead to the development of new therapeutic strategies.
Source	Rabbit
Specificity	The IL-6R Antibody can effectively detect the total levels of endogenous IL-6R. This means that it can accurately identify and quantify the levels of IL-6R in a given sample, providing valuable insights into the immune response and various inflammatory conditions. By using this antibody, researchers can gain a better understanding of how IL-6R functions in the body and how it may be targeted to treat a range of diseases.
Species Reactivity	Human;Mouse
Antigen	IL-6R
Application	The recommended dilutions for using the antibody to detect specific proteins in different assays are as follows: in Western blotting, use at dilutions of 1:500-1:2000; in immunohistochemistry, use at dilutions of 1:50-1:200; and in ELISA assays using peptides, use at dilutions of 1:20000-1:40000. These dilutions have been optimized and tested to provide the best results in each assay. It is important to note, however, that optimal dilutions may vary for different samples and experimental conditions, and it is recommended to perform titration experiments to determine the optimal dilution for individual applications.
Immunogen	A synthesized peptide derived from human IL-6R.
MW	52kD
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
Tissue specificity	Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.
Posttranslational modification	A short soluble form may also be released from the membrane by proteolysis.
Celluar localization	Extracellular region or secreted;Plasma Membrane;
UniPort	P08887