Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-LITAF Polyclonal Antibody#abs136869

Description

Catalog-specification	Delivery time	USD price
abs136869-100ug	1-2 Weeks	301.0
abs136869-50ug	1-2 Weeks	201.0

Regarding the pricing, please note that the amount mentioned is only for your reference. For the detailed pricing structure, we request you to kindly get in touch with our sales representative Vecent. Thank you. As an AI language model, I can create similar content by rephrasing the original text, but I cannot engage in dialogues like humans do. Therefore, please let me know if you need any further assistance in generating text.

Overview
catalog	abs136869
Description	Lipopolysaccharide activates monocytes and macrophages, resulting in the production of inflammatory mediators such as tumor necrosis factor-alpha (TNF-alpha). The lipopolysaccharide-induced TNF-alpha factor gene codes for a DNA-binding protein that can directly bind to the promoter region of the TNF-alpha gene, facilitating its expression. This gene's transcriptional activation is stimulated by the tumor suppressor p53 and is involved in the p53-induced apoptotic pathway. Charcot-Marie-Tooth disease type 1C (CMT1C) is caused by mutations in this gene, which may also contribute to the onset of extramammary Paget's disease (EMPD). This gene has several alternatively spliced transcript variants.
Other names	TNF alpha factor induced by lipopolysaccharide; CMT1C; FLJ38636; Tumor necrosis factor alpha factor induced by lipopolysaccharide; Tumor necrosis factor factor induced by lipopolysaccharide; Lipopolysaccharide-induced tumor necrosis factor alpha factor; Lipopolysaccharide-induced tumor necrosis factor-alpha factor; LITAF; Human lipopolysaccharide-induced TNF alpha factor; Human lipopolysaccharide-induced TNF-alpha factor; MGC116698; MGC116700; MGC116701; MGC125274; MGC125275; MGC125276; p53-induced gene 7 protein; p53-induced gene 7 protein; PIG 7; PIG7; Small integral membrane protein of lysosome/late endosome; TP53I7; Protein 7 induced by tumor protein p53.
Source	Rabbit
Specificity	The total LITAF levels can be accurately measured using the LITAF Antibody, which specifically detects endogenous LITAF levels. To provide a highly similar content rearranging the original information, it can be stated as follows: The endogenous levels of total LITAF can be effectively detected by utilizing the LITAF Antibody.
Species Reactivity	Human;Mouse;Rat
Antigen	LITAF
Application	The dilution range for WB is 1:500-1:2000, for IHC it is 1:50-1:200, and for ELISA (peptide) it is 1:20000-1:40000. Let's reorganize the information to create a highly similar content: For Western Blot (WB), the recommended dilution range is between 1:500 and 1:2000. This means that you should dilute your sample or antibody in a ratio of 1 part sample/antibody to 500-2000 parts of the diluent solution. In Immunohistochemistry (IHC), the optimal dilution range is 1:50-1:200. This indicates that you should dilute your primary antibody in a ratio of 1 part antibody to 50-200 parts of the diluent solution. For Enzyme-Linked Immunosorbent Assay (ELISA) using a peptide antigen, the suggested dilution range is 1:20000-1:40000. This implies that you should dilute your sample or antibody in a ratio of 1 part sample/antibody to 20000-40000 parts of the diluent solution. Please note that these dilution ranges serve as general recommendations and may vary depending on the specific experiment and target molecule. It is always recommended to optimize the dilution for your specific assay conditions to achieve the best results.
Immunogen	A synthesized peptide derived from human LITAF.
MW	17kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Plays a role in endosomal protein trafficking and in targeting proteins for lysosomal degradation (PubMed:23166352). Plays a role in targeting endocytosed EGFR and ERGG3 for lysosomal degradation, and thereby helps downregulate downstream signaling cascades (PubMed:23166352). Helps recruit the ESCRT complex components TSG101, HGS and STAM to cytoplasmic membranes (PubMed:23166352). Probably plays a role in regulating protein degradation via its interaction with NEDD4 (PubMed:15776429). May also contribute to the regulation of gene expression in the nucleus (PubMed:10200294, PubMed:15793005). Binds DNA (in vitro) and may play a synergistic role with STAT6 in the nucleus in regulating the expression of various cytokines (PubMed:15793005). May regulate the expression of numerous cytokines, such as TNF, CCL2, CCL5, CXCL1, IL1A and IL10 (PubMed:10200294, PubMed:15793005).
Tissue specificity	Ubiquitously and abundantly expressed. Expressed predominantly in the placenta, peripheral blood leukocytes, lymph nodes and spleen.
Posttranslational modification	Phosphorylated on tyrosine residues in response to EGF.
Celluar localization	Cytosol;Endosome;Golgi apparatus;Lysosome;Nucleus;Plasma Membrane;
UniPort	Q99732