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Rabbit Anti-IRF7 Polyclonal Antibody#abs136989

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Description

Catalog-specification	Delivery time	USD price
abs136989-100ug	1-2 Weeks	301.0
abs136989-50ug	1-2 Weeks	201.0

Overview
catalog	abs136989
Other names	This text refers to IMD39, also known as Interferon regulatory factor 7. There are several variations of this protein, including Interferon regulatory factor 7H, IRF 7, IRF 7A, IRF 7H, IRF7, IRF7A, IRF7B, and IRF7C. All of these variations of IRF7 play a role in regulating the body's immune response to viral infections. IRF7 is a transcription factor that activates the production of interferons, which are proteins that help protect cells from viruses. When a virus infects a cell, IRF7 becomes activated and triggers the production of interferons, which then signal nearby cells to prepare for a viral attack. In addition to its role in immune response, IRF7 is also involved in the development of certain immune cells, known as B cells. This suggests that IRF7 may play a role in the body's ability to produce long-lasting immunity. Overall, the variations of IRF7 play important roles in the body's immune system and response to viral infections. By activating the production of interferons and aiding in the development of immune cells, IRF7 helps protect the body from harmful viruses and pathogens.
Source	Rabbit
Specificity	Total IRF7 is effectively detected by the IRF7 Antibody, enabling the measurement of endogenous levels. The antibody specifically recognizes and binds to the entirety of IRF7 in a sample. This detection system provides valuable insights into the presence and quantity of IRF7, allowing researchers to study its function and regulation.
Species Reactivity	Human;Mouse;Rat
Antigen	IRF7
Application	The recommended dilutions for WB are 1:1000-3000, for IHC are 1:200, and for ELISA using a peptide are 1:20000-1:40000. Please note that the dilution factors may vary depending on the specific experiment and target protein. Generally, it is advisable to perform a titration experiment to determine the optimal dilution for each application.
Immunogen	A synthesized peptide derived from human IRF7.
MW	54 kDa
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography with SulfoLink™ Coupling Resin was employed to purify the antiserum. By rearranging the given information, a highly similar content can be generated.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the quality of the product, it should be stored at a temperature of -20 °C for a period of one year. It is important to avoid any repetitive freezing and thawing cycles as this can compromise the integrity of the product. Therefore, it is recommended that you carefully follow the storage instructions in order to ensure that the product remains stable and effective.
Storage buffer	The storage conditions for Rabbit IgG include keeping it in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C and can remain stable for up to 12 months from the date of receipt.
Target
Background	Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages.
Tissue specificity	Expressed predominantly in spleen, thymus and peripheral blood leukocytes.
Posttranslational modification	Acetylation inhibits its DNA-binding ability and activity.In response to a viral infection, phosphorylated on Ser-477 and Ser-479 by TBK1 and IKBKE1. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3. In TLR7- and TLR9-mediated signaling pathway, phosphorylated by IRAK1.TRAF6-mediated ubiquitination is required for IRF7 activation.Sumoylated by TRIM28, which inhibits its transactivation activity.
Celluar localization	Cytosol;Endosome;Nucleus;
UniPort	Q92985