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Rabbit Anti-HNMT Polyclonal Antibody#abs136502

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Description

Catalog-specification	Delivery time	USD price
abs136502-100ug	1-2 Weeks	301.0
abs136502-50ug	1-2 Weeks	201.0

Overview
catalog	abs136502
Description	Histamine, a neurotransmitter in mammals, undergoes metabolization through two main pathways: histamine N-methyltransferase, involving N(tau)-methylation, and diamine oxidase, involving oxidative deamination. The first enzyme responsible for histamine N(tau)-methylation is encoded by this gene and is predominantly present in the cytosol. It utilizes S-adenosyl-L-methionine as the methyl donor. In the central nervous system of mammals, the regulation of histamine's neurotransmitter activity relies heavily on N(tau)-methylation, as diamine oxidase is absent. Interestingly, a common genetic polymorphism affects the activity levels of this particular gene product within red blood cells. Additionally, there have been discovered multiple variations of transcript variants that are alternatively spliced and encode distinct proteins for this gene.
Other names	Histamine N methyltransferase, or EC 2.1.1.8, is also known as HMT or HNMT. It is an enzyme that catalyzes the transfer of a methyl group from S-adenosylmethionine to histamine, ultimately leading to the conversion of histamine to N-methylhistamine. HNMT has two isoforms: HNMT S1 and HNMT S2. In human, HNMT is encoded by the HNMT gene. Understanding the function of HNMT and its regulation may provide insights into the treatment of histamine-related disorders such as allergies and asthma.
Source	Rabbit
Specificity	The detection ability of HNMT Antibody is designed for determining the endogenous levels of total HNMT. Please note that the generated content will be rearranged to maintain the integrity of the original text information.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Sheep;Bovine;Horse;Rabbit
Antigen	HNMT
Application	The dilutions for Western Blot (WB) range from 1:500 to 1:2000, for Immunohistochemistry (IHC) range from 1:50 to 1:200, and for ELISA (peptide) range from 1:20000 to 1:40000. These dilution ranges represent the recommended dilutions for these assays based on the original information provided.
Immunogen	A synthesized peptide derived from human HNMT.
MW	33kDa
Properties
Concentration	1mg/ml
purification	By employing SulfoLink™ Coupling Resin, we performed peptide affinity chromatography to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine.
Celluar localization	Cytosol;Extracellular region or secreted;
UniPort	P50135