Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-HIF-3 Alpha Polyclonal Antibody#abs138077

Please note that the price mentioned above is only for your reference. For detailed pricing information, please reach out to our seller, Vecent. Western blot analysis HIF-3 alpha using COLO205 whole cell lysates This product is for research use only, not for use in diagnostic prodecures or in...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs138077-100ug	1-2 Weeks	301.0
abs138077-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, please reach out to our seller, Vecent.

Overview
catalog	abs138077
Other names	The protein MOP7, also known as basic-helix-loop-helix-PAS protein, bHLHe17, Class E basic helix-loop-helix protein 17, HIF 3A, HIF 3A4, HIF-3-alpha, HIF3 alpha, HIF3-alpha, HIF3-alpha-1, HIF3A, HIF3A_HUMAN, Hypoxia Inducible Factor 3 alpha, Hypoxia inducible factor 3 alpha subunit, Hypoxia inducible factor three alpha, Hypoxia-inducible factor 3-alpha, Inhibitory PAS domain protein, IPAS, Member of PAS protein 7, MOP7, and PAS domain-containing protein 7, is a versatile protein that plays a crucial role in hypoxia responses and gene regulation. It belongs to the basic helix-loop-helix-PAS family and is involved in the activation of various target genes under low oxygen conditions. MOP7 is an important member of the HIF transcription factor family, which regulates the expression of genes involved in adaptation to low oxygen levels. It contains a PAS domain, a structural motif found in proteins involved in sensing and responding to environmental signals. MOP7 interacts with other proteins to form heterodimers, which bind to specific DNA sequences and modulate gene expression. The hypoxia-inducible factor 3 alpha subunit, encoded by the HIF3A gene, is a key component of the HIF transcription factor complex. It has been identified as a negative regulator of the hypoxic response, acting as a modulator of HIF signaling pathways. HIF3A can inhibit the transcriptional activity of other HIF subunits and regulate the expression of target genes involved in angiogenesis, erythropoiesis, and metabolism. The complex regulatory network involving MOP7, HIF3A, and other HIF subunits allows for fine-tuned control of gene expression in response to varying oxygen levels. Understanding the functions and mechanisms of these proteins can provide valuable insights into the molecular basis of hypoxia adaptation and related diseases such as cancer and cardiovascular disorders. With further research, targeting MOP7 and HIF3A may hold promise for therapeutic interventions aimed at modulating hypoxia-induced signaling pathways.
Source	Rabbit
Specificity	Total HIF-3 alpha can be detected by the HIF-3 alpha Antibody, which specifically targets the endogenous levels of this protein.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Horse
Antigen	HIF-3 alpha
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A peptide that has been synthesized is based on the human HIF-3 alpha. This means that the peptide has been created by utilizing specific amino acid sequences found in this protein. The goal is to create a molecule that closely resembles HIF-3 alpha, which can be used in scientific research and medical applications. By generating this highly similar content, we can help others understand the importance and potential uses of this synthesized peptide, as well as the scientific principles behind its creation.
MW	72 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification of the antiserum was accomplished through peptide affinity chromatography. To ensure the antiserum was properly purified, this method was utilized and proved to be highly effective.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at -20 °C for one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Acts as a transcriptional regulator in adaptive response to low oxygen tension. Acts as a regulator of hypoxia-inducible gene expression (PubMed:11573933, PubMed:16126907, PubMed:19694616, PubMed:20416395, PubMed:21069422). Functions as an inhibitor of angiogenesis in hypoxic cells of the cornea. Plays a role in the development of the cardiorespiratory system. May also be involved in apoptosis (By similarity).
Tissue specificity	Expressed in vascular cells (at protein level) (PubMed:21069422). Expressed in kidney (PubMed:11573933, PubMed:19694616). Expressed in lung epithelial cells (PubMed:16775626). Expressed in endothelial cells (venous and arterial cells from umbilical cord and aortic endothelial cells) and in vascular smooth muscle cells (aorta) (PubMed:21069422). Strongly expressed in the heart, placenta, and skeletal muscle, whereas a weak expression profile was found in the lung, liver, and kidney (PubMed:12538644). Expressed weakly in cell renal cell carcinoma (CC-RCC) compared to normal renal cells (PubMed:16126907). Expression is down-regulated in numerous kidney tumor cells compared to non tumor kidney tissues (PubMed:16126907). Isoform 2 is expressed in heart, placenta, lung, liver, skeletal muscle and pancreas and in numerous cancer cell lines (PubMed:20416395). Isoform 3 and isoform 4 are weakly expressed in heart, placenta, lung, liver, skeletal muscle and pancreas (PubMed:20416395). Isoform 4 is expressed in fetal tissues, such as heart, brain, thymus, lung, liver, skeletal kidney and spleen (PubMed:20416395). Isoform 3 is weakly expressed in fetal tissues, such as liver and kidney (PubMed:20416395).
Posttranslational modification	In normoxia, hydroxylated on Pro-492 in the oxygen-dependent degradation domain (ODD) by prolyl hydroxylase(s) (PHD). The hydroxylated proline promotes interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation.Ubiquitinated; ubiquitination occurs in a VHL- and oxygen-dependent pathway and subsequently targeted for proteasomal degradation.
Celluar localization	Cytosol;Mitochondrion;Nucleus;Plasma Membrane;
UniPort	Q9Y2N7