Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-GTF2A1 Polyclonal Antibody#abs136799

Please note that the mentioned price is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent. In order to provide you with relevant and accurate details, it is advised not to rely on the content generated by ChapGPT as it may...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs136799-100ug	1-2 Weeks	301.0
abs136799-50ug	1-2 Weeks	201.0

Overview
catalog	abs136799
Description	The precise initiation of transcription on class II genes that contain a TATA box requires the sequential assembly of RNA polymerase II (POLR2A) and several general initiation factors. The process, as elucidated by DeJong and Roeder in 1993, involves the coordinated action of various components (PubMed 8224848). One crucial factor involved is TFIIA, which consists of three subunits when isolated from HeLa extracts: a 35-kD subunit, a 19-kD subunit, and a 12-kD subunit. It is through the concerted efforts of these molecular players that accurate transcription initiation is achieved.
Other names	The General transcription factor IIA subunit 1, also known as TFIIA-42 or TFIIA p19/35 subunit, is a protein that plays a crucial role in transcription initiation. It is encoded by the gtf2a1 gene, and its function is to stabilize the binding of the TATA-binding protein to the promoter region of genes. Additionally, TFIIA p19 subunit has been shown to contribute to the assembly of the pre-initiation complex by interacting with other transcription factors. TFIIA alpha p55, also known as TFIIA alpha p55 isoform 1 or TFIIAL, is another subunit of the TFIIA complex that consists of three subunits. It is important for the recruitment of RNA polymerase II to the promoter region and the formation of a stable pre-initiation complex. The glucose regulated protein 58kD pseudogene is a non-functional copy of the gtf2a1 gene, which has lost its ability to produce a functional protein due to mutations. Overall, the TFIIA complex plays a crucial role in transcription initiation, and its various subunits work together to ensure the efficient and accurate transcription of genes.
Source	Rabbit
Specificity	The detection of endogenous levels of total GTF2A1 is made possible by the GTF2A1 Antibody. This antibody specifically recognizes and targets the GTF2A1 protein present within an organism. This means that researchers can accurately measure the amount of GTF2A1 protein in biological samples, allowing for a better understanding of its role and function. By rearranging the information provided, we can convey the same message in a slightly different manner.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Zebrafish;Chicken;Rabbit;Xenopus;Dog;Horse;Bovine;Sheep;Pig
Antigen	GTF2A1
Application	The recommended dilutions for Western blotting (WB) range from 1:500 to 1:2000. For immunohistochemistry (IHC), it is suggested to use dilutions from 1:50 to 1:200. When performing immunofluorescence/immunocytochemistry (IF/ICC), dilutions of 1:100 to 1:500 are typically used. For ELISA assays using a peptide as the antigen, dilutions of 1:20000 to 1:40000 are commonly employed. These dilutions ensure optimal results and accurate detection of the target protein or antigen.
Immunogen	A synthesized peptide derived from human GTF2A1.
MW	19kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.
Posttranslational modification	The alpha and beta subunits are postranslationally produced from the precursor form by TASP1. The cleavage promotes proteasomal degradation.
Celluar localization	Cytosol;Nucleus;
UniPort	P52655