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Rabbit Anti-RHOBTB3 Polyclonal Antibody#abs140537

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Description

Catalog-specification	Delivery time	USD price
abs140537-100ug	1-2 Weeks	301.0
abs140537-50ug	1-2 Weeks	201.0

Overview
catalog	abs140537
Other names	The gene KIAA0878 encodes for a protein known as Rho related BTB domain containing protein 3, or RHOBTB 3. This protein belongs to the Rho family and contains a BTB domain. It is involved in various cellular processes such as cell migration and division. Overall, RHOBTB 3 is an important protein that plays a crucial role in regulating cellular activity.
Source	Rabbit
Specificity	The detection of total RHOBTB3 at endogenous levels is possible with the RHOBTB3 antibody. To create similar content, the information could be rearranged to state that the RHOBTB3 antibody is capable of identifying the full extent of endogenous RHOBTB3 levels.
Species Reactivity	Human;Rat
Antigen	RHOBTB3
Application	The recommended dilutions for WB, IHC, and ELISA (peptide) are as follows: WB 1:1000, IHC 1:200, and ELISA (peptide) 1:20000-1:40000. It is important to note these dilutions to ensure accurate and reliable results when using these techniques. With the proper dilution, these methods can be highly effective in detecting the presence of specific proteins in biological samples. It is always recommended to consult the protocol provided by the manufacturer or established literature when performing these assays.
Immunogen	A synthesized peptide derived from human RHOBTB3.
MW	70kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification process of the antiserum was accomplished via peptide affinity chromatography. To ensure high-quality purification, this method was employed to isolate and remove any impurities from the target protein.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is advised to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to multiple freeze/thaw cycles.
Storage buffer	This product contains rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. It is recommended to store this product at -20 °C to maintain its stability. The product is known to remain stable for up to 12 months from the date of receipt.
Target
Background	A Rab9-regulated ATPase is essential for the transportation of endosomes to the Golgi apparatus. Its main role is to facilitate the docking of transport vesicles at the Golgi complex. This ATPase is believed to contribute to the release of M6PRBP1/TIP47 from the vesicles, enabling their efficient fusion and docking at the Golgi. It exhibits a specific affinity for Rab9 and does not bind to other Rab proteins. The ATPase activity of this protein is naturally low due to autoinhibition, and this inhibition is alleviated by the presence of Rab9. To summarize, this protein is crucial for the proper transport and fusion of vesicles at the Golgi complex, with its function regulated by Rab9.
Tissue specificity	Ubiquitous. Highly expressed in neural and cardiac tissues, pancreas, placenta and testis.
Celluar localization	Cytosol;Extracellular region or secreted;Golgi apparatus;
UniPort	O94955