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Rabbit Anti-GLI1 Polyclonal Antibody#abs137009

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Description

Catalog-specification	Delivery time	USD price
abs137009-100ug	1-2 Weeks	301.0
abs137009-50ug	1-2 Weeks	201.0

Overview
catalog	abs137009
Other names	GLI, or Glioma-associated oncogene homolog 1, is a zinc finger protein that belongs to the GLI family. It is also known as GLI1 or Glioma associated oncogene 1. This protein plays a crucial role in the development and progression of various cancers, including gliomas. Its function is to regulate gene expression by binding to DNA and controlling gene transcription. As a member of the Kruppel family, GLI contains five C2H2-type zinc fingers which can bind to specific DNA sequences. Due to its importance in cancer development, GLI has been a promising target for cancer therapies. Overall, understanding the functions and mechanisms of GLI may provide important insights into cancer biology.
Source	Rabbit
Specificity	Total GLI1 levels can be detected by the GLI1 Antibody, which is capable of identifying endogenous levels of the protein. The antibody is designed to specifically target and detect the total amount of GLI1 present in a sample.
Species Reactivity	Human;Mouse;Rat
Antigen	GLI1
Application	For Western blotting, a suggested dilution range is between 1:1000 and 1:3000. For immunohistochemistry, a dilution of 1:200 is recommended. When conducting immunofluorescence, dilutions between 1:100 and 1:500 should be used. During ELISA, using peptide, it is suggested to dilute the sample between 1:20000 and 1:40000 for optimal results. These dilution ranges are based on previously established protocols and can be adjusted according to the specific requirements of the individual experiment. It is important to note that using appropriate dilutions can enhance the accuracy and sensitivity of the results, while reducing non-specific binding.
Immunogen	A synthesized peptide derived from human GLI1.
MW	119 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. The resulting process yielded a highly purified antiserum for further use.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality, it is recommended to store the product at -20 °C for a duration of one year. It is essential to avoid subjecting it to repeated cycles of freezing and thawing.
Storage buffer	This product is Rabbit IgG, which is available in phosphate buffered saline with a pH of 7.4. It also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol for improved stability. Ensure to store this product at -20 °C, and it will remain stable for up to 12 months from the date of receipt.
Target
Background	Acts as a transcriptional activator (PubMed:19706761, PubMed:10806483, PubMed:19878745, PubMed:24311597, PubMed:24217340). Binds to the DNA consensus sequence 5'-GACCACCCA-3' (PubMed:2105456, PubMed:8378770, PubMed:24217340). May regulate the transcription of specific genes during normal development (PubMed:19706761). May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling (PubMed:19706761). Plays a role in cell proliferation and differentiation via its role in SHH signaling (Probable).
Tissue specificity	Detected in testis (at protein level) (PubMed:2105456). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract (PubMed:19878745). Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues (PubMed:19706761).
Posttranslational modification	Phosphorylated in vitro by ULK3.Acetylation at Lys-518 down-regulates transcriptional activity. Deacetylated by HDAC1.
Celluar localization	Cytoskeleton;Cytosol;Nucleus;
UniPort	P08151