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Rabbit Anti-GCS-beta-2 Polyclonal Antibody#abs138072

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Description

Catalog-specification	Delivery time	USD price
abs130072-50ug	1-2 Weeks	201.0
abs130072-100ug	1-2 Weeks	301.0

Overview
catalog	abs130072
Description	Type II collagen, known as COL2A1, is a protein that plays a crucial role in the development of cartilaginous tissues. It's indispensable in the embryonic formation of the skeleton and enabling linear growth. This protein is also critical in the cartilage's ability to withstand compressive forces. COL2A1 belongs to the fibrillar collagen family and is made up of homotrimers of alpha 1(II) chains. Three isoforms of this protein are produced through alternative splicing in humans.
Other names	Type II collagen, also known as alpha 1 type II collagen, plays a crucial role in maintaining healthy cartilage. It is encoded by the COL2A1 gene and is essential for the structural integrity of various connective tissues. In addition to its role in cartilage, type II collagen is also involved in the formation and maintenance of other tissues like the inner ear and the vitreous humor of the eye. Mutations in the COL2A1 gene can result in various genetic disorders, including Stickler syndrome, spondyloepiphyseal dysplasia congenita (SEDC), and achondrogenesis type II. These conditions are characterized by abnormal skeletal development and joint abnormalities. Research has shown that type II collagen may have therapeutic potential in the treatment of certain musculoskeletal disorders, such as osteoarthritis. The use of type II collagen supplements has been suggested to support cartilage health and reduce joint pain and inflammation. Understanding the structure and function of type II collagen is crucial for advancing our knowledge of connective tissue disorders and developing new treatment strategies. Ongoing research in this field aims to unravel the complex mechanisms underlying collagen synthesis, assembly, and regulation, with the ultimate goal of improving the diagnosis, prevention, and management of collagen-related diseases.
Source	Rabbit
Specificity	The total Collagen II present in the body can be effectively detected using the Collagen II antibody that specifically targets the endogenous levels of this protein.
Species Reactivity	Human;Mouse;Rat
Antigen	Collagen II
Application	For western blotting, the recommended dilution range is between 1:500 to 1:3000. When it comes to immunohistochemistry, the suggested dilution is between 1:50 to 1:200. For IF/ICC, the recommended dilution range is between 1:100 to 1:500. As for ELISA (peptide), the suggested dilution is between 1:20000 to 1:40000. To summarize, these are the appropriate dilution ranges for each application.
Immunogen	A peptide that has been synthesized from the human Collagen II is what we have here. Would you like me to provide more information?
MW	140kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification of the antiserum was carried out through peptide affinity chromatography. Please create a similar content by rearranging the provided information, while maintaining its original meaning.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality, it is advised to keep the store temperature at -20 °C for a duration of one year. It is important to prevent the process of freezing and thawing from occurring repeatedly.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
Tissue specificity	Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte.
Posttranslational modification	Probably 3-hydroxylated on prolines by LEPREL1 (By similarity). Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2.
Celluar localization	Endoplasmic reticulum;Extracellular region or secreted;
UniPort	P02458