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Rabbit Anti-G6PD Polyclonal Antibody#abs135964

Please note that the price mentioned above is only for your reference. For the detailed price information, kindly get in touch with our seller, Vecent. Western blot analysis of extracts of variouscell lines, using G6PD antibody. This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs135964-50ug	1-2 Weeks	201.0
abs135964-100ug	1-2 Weeks	301.0

Please note that the price mentioned above is only for your reference. For the detailed price information, kindly get in touch with our seller, Vecent.

Overview
catalog	abs135964
Description	The pentose phosphate pathway is a crucial metabolic pathway, and its first and rate-limiting step is catalyzed by glucose-6-phosphate dehydrogenase (G6PD) (1). This enzyme plays a vital role in generating NADPH, which is essential for protecting cells against oxidative stress (1). Recent research has discovered a fascinating interaction between G6PD and the p53 protein. It has been observed that p53 can inhibit the activity of G6PD, leading to a suppression of glucose consumption through the pentose phosphate pathway (2). In cancer cells that carry p53 mutations, an interesting phenomenon occurs. The dysregulation caused by these mutations results in increased glucose consumption, which is primarily directed towards heightened biosynthesis. This redirection of glucose metabolism is critical for the rapid proliferation of cancer cells (2). Overall, G6PD and its interaction with p53 play a crucial role in the regulation of glucose metabolism and the control of oxidative stress. Understanding these mechanisms can provide valuable insights into the metabolic alterations that occur in cancer cells and potentially pave the way for new therapeutic strategies in combating cancer.
Other names	G6PD, also known as glucose 6 phosphate dehydrogenase, is an important enzyme involved in glucose metabolism. This enzyme plays a crucial role in the production of NADPH, a molecule necessary for the maintenance of proper cellular function. G6PD is encoded by the G6PD_HUMAN gene, also known as G6PD1. In yeast, the G6PD gene is referred to as Zwf1p, while in humans it is expressed in various tissues, including liver, kidney, and red blood cells. G6PD deficiency is a genetic disorder that affects approximately 400 million people worldwide. This condition is characterized by a decrease in the production of G6PD, leading to a range of symptoms such as jaundice, anemia, and susceptibility to infections. The severity of G6PD deficiency varies depending on the individual and can be triggered by certain drugs, infections, and foods. Research has shown that G6PD deficiency may have a protective effect against certain infectious diseases, such as malaria. However, this protection comes at a cost, as individuals with G6PD deficiency are more susceptible to oxidative stress and other health complications. It is essential to understand the role of G6PD in cellular metabolism and its impact on human health in order to develop effective treatments and interventions for G6PD deficiency.
Source	Rabbit
Specificity	The total levels of G6PD can be detected using G6PD Antibody, which specifically targets the endogenous G6PD.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Xenopus
Antigen	G6PD
Application	To get the best results for Western Blotting, you should dilute at a ratio of 1:500-1:2000. When it comes to Immunohistochemistry, you'll want to dilute at a ratio of 1:50-1:200. For ELISA using peptide, a dilution of 1:20000-1:40000 is recommended. These ratios are essential for ensuring accurate testing results in your experiments. Make sure to follow these guidelines carefully to achieve successful outcomes.
Immunogen	A synthesized peptide derived from human G6PD.
MW	59kDa
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography with SulfoLink™ Coupling Resin was employed to purify the antiserum. To obtain a highly comparable content, the original information is rearranged as requested.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 Â°C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 Â°C.Stable for 12 months from date of receipt.
Target
Background	Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
Tissue specificity	Isoform Long is found in lymphoblasts, granulocytes and sperm.
Posttranslational modification	Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
Celluar localization	Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;
UniPort	P11413