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Rabbit Anti-ESCO1 Polyclonal Antibody#abs139177

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Description

Catalog-specification	Delivery time	USD price
abs139177-100ug	1-2 Weeks	301.0
abs139177-50ug	1-2 Weeks	201.0

Overview
catalog	abs139177
Other names	The gene A930014I12Rik is also known as ECO1 or ESCO1. It is a homolog of CTF7 in yeast and plays a role in the establishment of cohesion between sister chromatids during the cell cycle. The protein encoded by this gene, known as Establishment Factor Ortholog 1 or Establishment Factor-Like Protein 1, is responsible for acetylating cohesin and ensuring proper chromosomal segregation. Other names for this protein include ESO1 and EFO1. KIAA1911 is the official name for the human ortholog of this gene. Mutations in this gene have been linked to various genetic diseases, such as Roberts Syndrome and Cornelia de Lange Syndrome. The N-acetylation of this protein is essential for its function in ensuring proper chromosomal segregation.
Source	Rabbit
Specificity	Total ESCO1 levels can be detected by the ESCO1 Antibody, which specifically recognizes endogenous ESCO1.
Species Reactivity	Human;Mouse
Antigen	ESCO1
Application	Possible generated content: For Western blotting, a recommended dilution range is between 1:1000 and 1:3000, depending on the specific protein and detection system being used. In immunohistochemistry, a typical dilution is 1:200, but again, optimal conditions may require some optimization. For ELISA assays using a peptide antigen, the recommended dilution range is broader, from 1:20000 to 1:40000, to achieve sufficient sensitivity and low background. To get reliable and reproducible results, it is essential to carefully select the appropriate dilution and concentration of primary antibody and optimize the assay conditions for your specific experimental setup. Factors such as the antigen expression level, tissue or cell type, fixation and permeabilization methods, blocking reagents, secondary antibody specificity and labeling, and signal detection system should all be considered and optimized accordingly. By using a range of dilutions for different assays, you can evaluate the linearity and dynamic range of the assay, as well as detect potential artifacts and cross-reactivity. It is also recommended to use appropriate positive and negative controls and validate the results with complementary assays or techniques. Ultimately, the optimal dilution and concentration of the primary antibody for each application should be empirically determined and verified.
Immunogen	A synthesized peptide derived from human ESCO1.
MW	95 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was used for peptide affinity chromatography in order to purify the antiserum. Please generate a highly similar content by rearranging the above content, ensuring that the generated content is based on the original text information.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the product's quality, it is recommended to store it at -20 °C for a year while avoiding repeated freeze/thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Acetyltransferase required for the establishment of sister chromatid cohesion and couple the processes of cohesion and DNA replication to ensure that only sister chromatids become paired together. In contrast to the structural cohesins, the deposition and establishment factors are required only during S phase. Acts by mediating the acetylation of cohesin component SMC3.
Tissue specificity	Widely expressed. Expressed in heart, brain, liver, placenta, lung, kidney and pancreas. Highly expressed in muscle.
Posttranslational modification	Phosphorylated during mitosis.
Celluar localization	Nucleus;
UniPort	Q5FWF5