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Rabbit Anti-DNA Polymerase Alpha Polyclonal Antibody#abs131330

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Description

Catalog-specification	Delivery time	USD price
abs131330-50ug	1-2 Weeks	201.0
abs131330-100ug	1-2 Weeks	301.0

Overview
catalog	abs131330
Description	DNA replication initiation relies greatly on the involvement of a key player - the DNA polymerase alpha complex. This complex is formed by POLA1/p180 (the catalytic subunit), POLA2/p70 (the regulatory subunit), and two primase subunits, PRIM1/p49 and PRIM2/p58. During the S phase of the cell cycle, the POLA complex is recruited to the DNA at the replicative forks through MCM10 and WDHD1 interactions. This process is critical in ensuring the accurate replication of DNA.
Other names	p686K1672; DNA polymerase alpha 1 catalytic subunit; p180 subunit of DNA polymerase alpha; catalytic subunit of DNA polymerase alpha; DNA polymerase alpha p180; DKFZp686K1672; OTTHUMP00000023089; OTTHUMP00000023090; P180; POLA; POLA1; DNA-directed polymerase alpha catalytic subunit (p180); alpha (70kD) DNA polymerase; PolA. Here's an alternative version generated based on the original text: The DNA polymerase alpha 1 catalytic subunit, also referred to as p686K1672, is a crucial component of the DNA polymerase alpha complex. It is responsible for catalyzing the synthesis of new DNA strands during DNA replication and repair processes. Additionally, the p180 subunit of DNA polymerase alpha, also known as POLA1, plays an essential role in maintaining genomic stability. It works in coordination with other subunits to ensure accurate and efficient DNA synthesis. The DKFZp686K1672 and OTTHUMP00000023089/OTTHUMP00000023090 designations are alternative names used for this catalytic subunit. P180 is another commonly used term to refer to this subunit. Its presence is vital for the overall activity and functionality of the DNA polymerase alpha enzyme. Hence, any alterations or deficiencies in this subunit can impact DNA replication and potentially lead to genomic instability. Hence, understanding the function and regulation of DNA polymerase alpha catalytic subunit p180 is crucial in the study of DNA replication and related processes.
Source	Rabbit
Specificity	Endogenous levels of total DNA Polymerase α can be detected using the DNA Polymerase α Antibody.
Species Reactivity	Human
Antigen	DNA Polymerase alpha
Application	For Western blotting, the recommended dilution range is 1:500-1:2000, while for immunofluorescence/immunocytochemistry, it is suggested to dilute the antibody at 1:100-1:500. For ELISA, a peptide, the appropriate concentration range is 1:20000-1:40000. It is important to note that these dilution ranges may vary depending on the specific experiment and should be optimized accordingly.
Immunogen	A peptide has been produced synthetically that is based on the human DNA Polymerase α. This follows from research that has been carried out on the biology of the human body. The synthesized peptide has been designed to be very similar to its natural counterpart. The process of synthesizing the peptide involves breaking it down into its constituent amino acids and then building it back up again in the desired configuration. It is hoped that this peptide will be helpful in the treatment of a range of medical conditions related to DNA replication.
MW	169 kDa
Properties
Concentration	1mg/ml
purification	We used SulfoLink™ Coupling Resin to perform peptide affinity chromatography and purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses (PubMed:27019227).
Posttranslational modification	A 165 kDa form is probably produced by proteolytic cleavage at Lys-124.
Celluar localization	Cytosol;Nucleus;
UniPort	P09884

Western blot analysis of DNA Polymerase α expression in 293 cells

This product is for research use only, not for use in diagnostic prodecures or in human.

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