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Rabbit Anti-CLK4 Polyclonal Antibody#abs138165

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Description

Catalog-specification	Delivery time	USD price
abs138165-100ug	1-2 Weeks	301.0
abs138165-50ug	1-2 Weeks	201.0

Overview
catalog	abs138165
Other names	CLK4, also known as CDC-like kinase 4, is a protein serine/threonine kinase that plays a crucial role in various cellular processes. The AI987988 sequence corresponds to the human CLK4 gene, which is also referred to as DKFZp686A20267. This gene encodes a dual specificity protein kinase that exerts its catalytic activity on both serine and threonine residues. CLK4 is structurally similar to CDC kinase and plays a significant role in cell cycle regulation and splicing events. It is involved in the phosphorylation of SR proteins, which are essential for pre-mRNA splicing. This kinase is highly conserved across species, including mice, where it is encoded by the Otummusp00000005756, Otummusp00000005757, and Otummusp00000041113 genes. In mice, this kinase is referred to as RP23-9N7.1. Understanding the function and regulation of CLK4 is vital for comprehending various cellular processes and their dysregulation in diseases. Further research on this protein can provide insights into potential therapeutic targets for the treatment of human disorders related to cell cycle control and splicing.
Source	Rabbit
Specificity	The endogenous levels of total CLK4 can be accurately detected using the CLK4 Antibody. Reordering the original content, a highly similar paragraph is generated.
Species Reactivity	Human;Mouse
Predictive reaction species	Sheep;Bovine;Dog;Horse;
Antigen	CLK4
Application	Here are a few possible variations of the original text: - To detect our protein of interest, we recommend using Western Blotting at a dilution of 1:1000-3000. For immunofluorescence or immunocytochemistry experiments, a dilution of 1:100-1:500 should suffice. For ELISA assays with the peptide antigen, we suggest using a dilution range of 1:20000-1:40000. - If you want to visualize your target protein, we suggest trying Western Blotting with a dilution range of 1:1000-3000. For staining experiments, such as IF or ICC, you can use a dilution of 1:100-1:500. In ELISA assays with the peptide antigen, a dilution range of 1:20000-1:40000 is recommended. - When performing experiments to detect our protein of interest, we suggest using Western Blotting with a dilution range of 1:1000-3000. For imaging-based techniques like IF or ICC, a dilution of 1:100-1:500 should be sufficient. In ELISA assays using the peptide as the antigen, we recommend using a dilution range of 1:20000-1:40000. Note: As a language model AI, I am unable to communicate with humans. I am only capable of generating text based on prompts given to me.
Immunogen	A synthesized peptide derived from human CLK4.
MW	57 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification of the antiserum was performed through peptide affinity chromatography. An efficient method, the process involved isolating the specific peptide that the antiserum is directed against and purifying it to a high level of specificity. The resulting purified antiserum is well-suited for a range of biological applications, and its quality and purity are closely monitored throughout the process to ensure peak efficacy and performance.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve its quality, the product should be stored at a temperature of -20 °C for a duration of one year. It is essential to prevent the product from undergoing multiple cycles of freezing and thawing.
Storage buffer	pH 7.4 Rabbit IgG solution is prepared in phosphate buffered saline containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This solution should be stored at -20 °C and remains stable for 12 months from the date of receipt.
Target
Background	Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates SRSF1 and SRSF3. Required for the regulation of alternative splicing of MAPT/TAU. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
Tissue specificity	Expressed in liver, kidney, heart, muscle, brain and endothelial cells.
Posttranslational modification	Autophosphorylates on all three types of residues.
Celluar localization	Nucleus;
UniPort	Q9HAZ1