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Rabbit Anti-SCNN1A Polyclonal Antibody#abs138719

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Description

Catalog-specification	Delivery time	USD price
abs138719-100ug	1-2 Weeks	301.0
abs138719-50ug	1-2 Weeks	201.0

Overview
catalog	abs138719
Other names	The Alpha ENaC 2, also known as Alpha NaCH, Alpha-ENaC, or Alpha-NaCH, is a subunit of the amiloride-sensitive epithelial sodium channel. This subunit, encoded by the SCNN1A gene, plays a key role in sodium transport across epithelial cells. It is also referred to as the Epithelial Na+ channel subunit alpha or the Nonvoltage-gated sodium channel 1 subunit alpha. The Amiloride-sensitive sodium channel subunit alpha is highly expressed in the kidneys, lungs, and colon. It is an important target for diuretics, which help to reduce fluid levels in the body by inhibiting its activity. Mutations in the SCNN1A gene have been linked to a rare genetic disorder known as Liddle syndrome, which results in excessive sodium reabsorption. The Alpha ENaC is a vital component of the body's sodium balance, as it regulates sodium absorption in the kidney and other epithelial tissues. This subunit has been extensively studied for its role in various disease states, including hypertension, heart failure, and cystic fibrosis. Understanding the function and regulation of the Alpha ENaC is critical for the development of effective therapies for these conditions.
Source	Rabbit
Specificity	The endogenous levels of total SCNN1A can be detected using the SCNN1A Antibody. Let me rearrange the content to generate a highly similar statement: The SCNN1A Antibody is capable of detecting the total SCNN1A levels present naturally in the body.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Pig;Dog;Horse;
Antigen	SCNN1A
Application	Here are some possible rearrangements of the original content: - When performing western blotting, it's recommended to use a dilution of 1:1000-3000 for the primary antibody. For immunohistochemistry, a dilution of 1:100-200 should be sufficient. Finally, when using a peptide-based ELISA assay, a dilution of 1:20000-1:40000 is appropriate. - To get optimal results with western blotting, experiment with primary antibody dilutions between 1:1000-3000. For immunohistochemistry, try dilutions of 1:100-200. And if you're using a peptide-based ELISA, a dilution range of 1:20000-1:40000 has been suggested. - If you're wondering what dilutions to use for your western blotting, immunohistochemistry and peptide-based ELISA experiments, here are some recommendations: 1:1000-3000 for western blotting, 1:100-200 for immunohistochemistry, and 1:20000-1:40000 for ELISA using a peptide substrate.
Immunogen	A synthesized peptide derived from human SCNN1A.
MW	76kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography to purify the antiserum. Can you please generate content in a highly similar manner but rearrange the information from the original text?
Clonality	Polyclonal Antibody
Stability & Storage	Ensure that the product is stored at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to multiple freeze/thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and eccrine sweat glands. Also plays a role in taste perception.
Tissue specificity	Expressed in the female reproductive tract, from the fimbrial end of the fallopian tube to the endometrium (at protein level) (PubMed:22207244). Expressed in kidney (at protein level). In the respiratory tract, expressed in the bronchial epithelium (at protein level). Highly expressed in lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta (PubMed:22207244). in skin, expressed in keratinocytes, melanocytes and Merkel cells of the epidermal sub-layers, stratum basale, stratum spinosum and stratum granulosum (at protein level) (PubMed:28130590). Expressed in the outer root sheath of the hair follicles (at protein level) (PubMed:28130590). Detected in both peripheral and central cells of the sebaceous gland (at protein level) (PubMed:28130590). Expressed by eccrine sweat glands (at protein level) (PubMed:28130590). In skin, also expressed by arrector pili muscle cells and intradermal adipocytes (PubMed:28130590). Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart (PubMed:9575806).
Posttranslational modification	Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation.ENaC cleavage by furin, and subsequently by prostasin (PRSS8), leads to a stepwise increase in the open probability of the channel as a result of release of the alpha and gamma subunit inhibitory tracts, respectively. Interaction of ENaC subunit SCNN1B with BPIFA1 protects ENaC against proteolytic activation.N-glycosylated.
Celluar localization	Extracellular region or secreted;Plasma Membrane;
UniPort	P37088