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Rabbit Anti-CLIP1 Polyclonal Antibody#abs140955

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Description

Catalog-specification	Delivery time	USD price
abs140955-100ug	1-2 Weeks	301.0
abs140955-50ug	1-2 Weeks	201.0

Overview
catalog	abs140955
Other names	CAP-Gly domain-containing linker protein 1, also known as CLIP-170 or cytoplasmic linker protein 1, is a protein that plays a crucial role in cellular processes. It is encoded by the CLIP1 gene and is expressed in various tissues. The CAP-Gly domain of CLIP-170 enables its interaction with microtubules, allowing it to function as a linker between the microtubule network and other cellular structures. This protein is involved in processes such as mitotic spindle formation, vesicle transport, and cell migration. CLIP-170 has been implicated in several diseases and conditions. For instance, its abnormal expression has been observed in certain cancer types, suggesting its potential role in tumor progression. Additionally, mutations in CLIP1 have been associated with disorders like intellectual disability and autism spectrum disorders. Research on CLIP-170 continues to uncover its diverse functions and mechanisms of action. Understanding its role in cellular processes may provide insights into novel therapeutic strategies for various diseases. The study of this protein is an active area of investigation in the field of molecular biology and genetics.
Source	Rabbit
Specificity	The expression of total CLIP1 can be detected by the CLIP1 Antibody, which specifically binds to endogenous levels of CLIP1.
Species Reactivity	Human;Mouse;Rat
Antigen	CLIP1
Application	For western blotting, dilutions of 1:500 to 1:1000 are recommended. When performing immunofluorescence or immunocytochemistry, a dilution range of 1:100 to 1:500 is suitable. For ELISA using peptides, a dilution of 1:20000 to 1:40000 is optimal. It is important to adhere to these dilution recommendations in order to achieve accurate and reliable results in your experiments.
Immunogen	A synthesized peptide.
MW	161 KD
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized in the purification process of the antiserum through peptide affinity chromatography. Let's rearrange the given content to generate a highly similar version: "To purify the antiserum, a peptide affinity chromatography method was employed, in which SulfoLink™ Coupling Resin played a vital role."
Clonality	Polyclonal Antibody
Stability & Storage	To ensure the longevity of the product, it is advised to store it at a temperature of -20 °C for a period of one year. It is important to avoid exposing the product to repeated freeze/thaw cycles as this could compromise its integrity. Take care to follow these instructions to maintain the quality of the product.
Storage buffer	The storage solution for rabbit IgG is composed of phosphate buffered saline, with a pH of 7.4, 150mM NaCl, and 0.02% sodium azide; containing 50% glycerol. It's important to store it at a temperature of -20°C to preserve its efficacy, and it remains stable for a period of 12 months from the date of receipt.
Target
Background	Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking.
Tissue specificity	Detected in dendritic cells (at protein level). Highly expressed in the Reed-Sternberg cells of Hodgkin disease.
Posttranslational modification	Phosphorylated. Phosphorylation induces conformational changes by increasing the affinity of the N-terminus for C-terminus, resulting in inhibition of its function thus decreasing its binding to microtubules and DCTN1. Exhibits a folded, autoinhibited conformation when phosphorylated and an open conformation when dephosphorylated with increased binding affinity to microtubules and DCTN1. Phosphorylation regulates its recruitment to tyrosinated microtubules and the recruitment of vesicular cargo to microtubules in neurons (By similarity). Phosphorylation by MTOR may positively regulate CLIP1 association with microtubules (PubMed:12231510).
Celluar localization	Cytoskeleton;Cytosol;Endosome;Nucleus;
UniPort	P30622