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Rabbit Anti-CEP135 Polyclonal Antibody#abs140995

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Description

Catalog-specification	Delivery time	USD price
abs140995-100ug	1-2 Weeks	301.0
abs140995-50ug	1-2 Weeks	201.0

Overview
catalog	abs140995
Other names	Protein 135 kDa of the centrosome, commonly known as Centrosomal protein 135kDa or Cep135, is a vital component found in the centrosome. It plays an important role in the organization and function of this cellular organelle. This protein, with a molecular weight of 135 kDa, is also referred to as Centrosomal protein 4 (Centrosome protein 4) or CEP4. Cep135 is crucial for the proper assembly and maintenance of the centrosome, a microtubule-organizing center existing in human cells. It contributes to the regulation of cell division and the formation of the mitotic spindle, which is essential for proper chromosome segregation during cell division. Furthermore, the protein is involved in microtubule organization and stabilization, playing a pivotal role in cellular processes such as cell motility and intracellular transportation. The designation CP135_HUMAN refers to the protein's identification as a 135-kDa centrosome protein found in humans. The detailed understanding of Cep135's functions and interactions with other centrosomal proteins is critical for unraveling the complex processes occurring within the centrosome and their implications in various cellular activities.
Source	Rabbit
Specificity	The CEP135 Antibody is capable of detecting total endogenous levels of CEP135. The content can be rephrased by stating that the antibody has the ability to recognize and detect the complete amount of CEP135 in its natural form.
Species Reactivity	Human;Mouse
Predictive reaction species	Rabbit;Sheep;Dog;Horse;
Antigen	CEP135
Application	For Western Blotting, the recommended dilution is between 1:500 to 1:1000. If you are doing Immunofluorescence/Immunocytochemistry, dilute between 1:100 to 1:500. For ELISA with a peptide, the recommended dilution is between 1:20000 to 1:40000. Please note that these dilutions are based on the original text information and should be followed closely for best results.
Immunogen	A synthesized peptide.
MW	134 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, a peptide affinity chromatography process was employed to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage conditions, it is recommended to store the product at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this may affect its quality and efficacy. Please follow these guidelines to maintain the product's stability and integrity throughout its shelf life.
Storage buffer	The rabbit IgG solution comes in a phosphate buffered saline, pH 7.4, with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol. Keep it stored at -20 °C for maximum stability, and it should remain stable for up to 12 months from the date of receipt.
Target
Background	Centrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole.
Celluar localization	Cytoskeleton;Cytosol;
UniPort	Q66GS9